Brasil
| Artigo
Role of Paracoccidioides brasiliensis cell wall fraction containing beta-glucan in tumor necrosis factor-alpha production by human monocytes: correlation with fungicidal activity
dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | Universidade de São Paulo (USP) | |
dc.date.accessioned | 2014-05-20T15:26:53Z | |
dc.date.accessioned | 2022-10-05T16:40:06Z | |
dc.date.available | 2014-05-20T15:26:53Z | |
dc.date.available | 2022-10-05T16:40:06Z | |
dc.date.created | 2014-05-20T15:26:53Z | |
dc.date.issued | 2002-08-01 | |
dc.identifier | Medical Mycology. Oxford: B I O S Scientific Publishers Ltd, v. 40, n. 4, p. 377-382, 2002. | |
dc.identifier | 1369-3786 | |
dc.identifier | http://hdl.handle.net/11449/36955 | |
dc.identifier | 10.1080/714031128 | |
dc.identifier | WOS:000177706100004 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3908572 | |
dc.description.abstract | The polysaccharide fraction of Paracoccidioides brasiliensis mycelial cell wall (F1 fraction), the active component of which is composed of beta-glucan, was investigated in regard to the activation of human monocytes for fungal killing. The cells were primed with interferon-gamma (IFN-gamma) or F1 (100 and 200 mug ml(-1)) or F1 (100 and 200 mug ml(-1)) plus IFN-gamma for 24 h and then evaluated for H2O2 release. In other experiments, the cells were pretreated with the same stimuli, challenged with a virulent strain of P. brasiliensis and evaluated for fungicidal activity and levels of tumor necrosis factor (TNF-alpha) in the supernatants. F1 increased the levels of H2O2 in a similar manner to IFN-gamma. However, a synergistic effect between these two activators was not detected. on the contrary, a significant fungicidal activity was only obtained after priming with IFN-gamma plus F1. This higher activity was associated with high levels of TNF-alpha in the supernatants of the cocultures. Overall, P. brasiliensis F1 fraction induced human monocytes to release relatively high levels of TNF-alpha, which, in combination with IFN-gamma, is responsible for the activation of human monocytes for effective killing of P. brasiliensis. | |
dc.language | eng | |
dc.publisher | B I O S Scientific Publishers Ltd | |
dc.relation | Medical Mycology | |
dc.relation | 2.799 | |
dc.relation | 0,973 | |
dc.rights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | beta-glucan | |
dc.subject | fungicidal activity | |
dc.subject | monocyte | |
dc.subject | Paracoccidioides brasiliensis | |
dc.subject | TNF | |
dc.title | Role of Paracoccidioides brasiliensis cell wall fraction containing beta-glucan in tumor necrosis factor-alpha production by human monocytes: correlation with fungicidal activity | |
dc.type | Artigo |