Artigo
Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa
Fecha
2003-02-01Registro en:
Journal of Bacteriology. Washington: Amer Soc Microbiology, v. 185, n. 3, p. 1018-1026, 2003.
0021-9193
10.1128/JB.185.3.1018-1026.2003
WOS:000180834300035
WOS000180834300035.pdf
7179273060624761
6676176632132637
0147241723612464
0165348738208319
0000-0003-4524-954X
Autor
Universidade de São Paulo (USP)
Inst Agron Campinas
Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
Inst Ludwig Pesquisa Canc
Univ Mogi das Cruzes
Universidade Federal de São Carlos (UFSCar)
Universidade Federal de Mato Grosso do Sul (UFMS)
USDA ARS
Resumen
Xylella fastidiosa is a xylem-dwelling, insect-transmitted, gamma-proteobacterium that causes diseases in many plants, including grapevine, citrus, periwinkle, almond, oleander, and coffee. X. fastidiosa has an unusually broad host range, has an extensive geographical distribution throughout the American continent, and induces diverse disease phenotypes. Previous molecular analyses indicated three distinct groups of X.fastidiosa isolates that were expected to be genetically divergent. Here we report the genome sequence of X. fastidiosa (Temecula strain), isolated from a naturally infected grapevine with Pierce's disease (PD) in a wine-grape-growing region of California. Comparative analyses with a previously sequenced X.fastidiosa strain responsible for citrus variegated chlorosis (CVC) revealed that 98% of the PD X.fastidiosa Temecula genes are shared with the CVC X. fastidiosa strain 9a5c genes. Furthermore, the average amino acid identity of the open reading frames in the strains is 95.7%. Genomic differences are limited to phage-associated chromosomal rearrangements and deletions that also account for the strain-specific genes present in each genome. Genomic islands, one in each genome, were identified, and their presence in other X.fastidiosa strains was analyzed. We conclude that these two organisms have identical metabolic functions and are likely to use a common set of genes in plant colonization and pathogenesis, permitting convergence of functional genomic strategies.