Artigo
Comparison of hexamethyidisilazane and critical point drying treatments for SEM analysis of anaerobic biofilms and granular sludge
Date
2003-01-01Registration in:
Journal of Electron Microscopy. Oxford: Oxford Univ Press, v. 52, n. 4, p. 429-433, 2003.
0022-0744
10.1093/jmicro/52.4.429
WOS:000185860700011
Author
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
Abstract
We present a fast procedure for scanning electron microscopy (SEM) analysis in which hexamethyldisilazane (HMDS) solvent, instead of the critical point drying, is used to remove liquids from a microbiological specimen. The results indicate that the HMDS solvent is suitable for drying samples of anaerobic cells for examination by SEM and does not cause cell structure disruption.