Artigo
In vitro effects of endodontic irrigants on endotoxins in root canals
Fecha
2007-07-01Registro en:
Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology. New York: Mosby-elsevier, v. 104, n. 1, p. 135-142, 2007.
1079-2104
10.1016/j.tripleo.2006.11.037
WOS:000248103500023
4716676611330311
0053567153623569
6543563161403421
0000-0002-2416-2173
Autor
Universidade Estadual Paulista (Unesp)
Resumen
Objective. The objective of this study was to evaluate the effects of endodontic irrigants on endotoxins in root canals.Study design. Ninety-eight single-root human teeth were used. Escherichia coli endotoxin was inoculated into 84 root canals. All root canals were enlarged and assigned to 7 groups (n = 14), according to solution used. Group 1 (G1): 2.5% NaOCl; G2: 5.25% NaOCl; G3: 2% chlorhexidine; G4: 0.14% calcium hydroxide; G5: polymyxin B; G6: positive control, saline solution; G7: negative control (no endotoxin). Two samplings of root canal were accomplished: immediate and after 7 days. Detoxification of endotoxin was evaluated by Limulus assay and antibody production in B-lymphocyte culture. Results were analyzed by Kruskal-Wallis/Dunn and ANOVA/Tukey.Results. At the immediate and second samplings, groups G4, G5, and G7 presented the best results, significantly different from groups G1, G2, G3, and G6 (P = .05).Conclusions. Calcium hydroxide and polymyxin B detoxified endotoxin in root canals and altered properties of LPS to stimulate the antibody production by B-lymphocytes. Sodium hypochlorite and chlorhexidine did not detoxify endotoxin.