dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | Universidade de São Paulo (USP) | |
dc.date.accessioned | 2014-05-20T15:19:51Z | |
dc.date.accessioned | 2022-10-05T16:02:26Z | |
dc.date.available | 2014-05-20T15:19:51Z | |
dc.date.available | 2022-10-05T16:02:26Z | |
dc.date.created | 2014-05-20T15:19:51Z | |
dc.date.issued | 2007-08-01 | |
dc.identifier | Cytokine. London: Academic Press Ltd Elsevier B.V. Ltd, v. 39, n. 2, p. 130-137, 2007. | |
dc.identifier | 1043-4666 | |
dc.identifier | http://hdl.handle.net/11449/31237 | |
dc.identifier | 10.1016/j.cyto.2007.06.009 | |
dc.identifier | WOS:000250913700006 | |
dc.identifier | 5333250355049814 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3904039 | |
dc.description.abstract | This study evaluated the effects of bFGF and TGF-beta, individually and combined, on cell proliferation and collagen metabolism. Primary human periodontal ligament cells were stimulated with two concentrations (I and 10 ng/ml) of each growth factor, both individually and combined. Proliferation was determined by a commercial biochemical assay. Real time RT-PCR determined gene expression of NMP-1 and -2, collagen types I and III, TIMP-1, -2 and -3. Autocrine effects on synthesis of bFGF and TGF-beta were evaluated by ELISA. Only TGF-beta, either isolated or associated with bFGF, significantly increased cell proliferation. TGF-beta had anabolic effects, increasing expression of type I and III collagen as well as of TIMPs, whereas bFGF had opposite effects. When bFGF and TGF-beta were associated, the anabolic effects prevailed. Synthesis of TGF-beta was induced only by the association of lower concentrations of the growth factors, whereas there was a dose-dependent production of bFGF. It is concluded that bFGF had a predominantly catabolic effect, and TGF-beta exerted an anabolic effect on hPDL cells. (c) 2007 Elsevier Ltd. All rights reserved. | |
dc.language | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation | Cytokine | |
dc.relation | 3.514 | |
dc.relation | 1,433 | |
dc.rights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | human periodontal ligament cells | |
dc.subject | transforming growth factor beta | |
dc.subject | basic fibroblast growth factor | |
dc.subject | collagen | |
dc.subject | matrix metalloproteases | |
dc.subject | tissue inhibitor of matrix metalloproteases | |
dc.subject | periodontal regeneration | |
dc.title | Opposite effects of bFGF and TGF-beta on collagen metabolism by human periodontal ligament fibroblasts | |
dc.type | Artigo | |