Artigo
Chromosomal imbalances detected in primary bone tumors by comparative genomic hybridization and interphase fluorescence in situ hybridization
Fecha
2003-01-01Registro en:
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 26, n. 2, p. 107-113, 2003.
1415-4757
10.1590/S1415-47572003000200001
S1415-47572003000200001
S1415-47572003000200001.pdf
2259986546265579
6882035465809248
Autor
Universidade de São Paulo (USP)
University Health Network Princess Margaret Hospital Ontario Cancer Institute
University of Toronto Department of Medical Biophysics
University of Toronto Department of Laboratory Medicine and Pathobiology
Universidade Estadual Paulista (Unesp)
Resumen
We applied a combination of comparative genomic hybridization (CGH) and fluorescence in situ hybridization (FISH), to characterize the genetic aberrations in three osteosarcomas (OS) and one Ewing's sarcoma. CGH identified recurrent chromosomal losses at 10p14-pter and gains at 8q22.3-24.1 in OS. Interphase FISH allowed to confirm 8q gain in two cases. A high amplification level of 11q12-qter was detected in one OS. The Ewing's sarcoma showed gain at 1p32-36.1 as the sole chromosome alteration. These studies demonstrate the value of molecular cytogenetic methods in the characterization of recurrent genomic alterations in bone tumor tissue.