dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | Universidade de São Paulo (USP) | |
dc.date.accessioned | 2014-05-20T14:20:14Z | |
dc.date.accessioned | 2022-10-05T15:20:03Z | |
dc.date.available | 2014-05-20T14:20:14Z | |
dc.date.available | 2022-10-05T15:20:03Z | |
dc.date.created | 2014-05-20T14:20:14Z | |
dc.date.issued | 2010-06-01 | |
dc.identifier | Bioresource Technology. Oxford: Elsevier B.V., v. 101, n. 12, p. 4251-4260, 2010. | |
dc.identifier | 0960-8524 | |
dc.identifier | http://hdl.handle.net/11449/26077 | |
dc.identifier | 10.1016/j.biortech.2010.01.041 | |
dc.identifier | WOS:000276374500001 | |
dc.identifier | 4484083685251673 | |
dc.identifier | 1308042794786872 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3899112 | |
dc.description.abstract | The ability to carry out geranylations on aromatic substrates using enzymatic extracts from the leaves of Piper crassinervium (Piperaceae) was evaluated. A literature analysis pointed out its importance as a source of prenylated bioactive molecules. The screening performed on aromatic acceptors (benzoic acids, phenols and phenylpropanoids) including geranyl diphosphate as prenyl donor, showed the biotransformation of the 3,4-dihydroxybenzoic acid by the crude extract, and the p-hydroxybenzoic acid by both the microsomal fraction and the crude extract, after treating leaves with glucose. The analysis of the products allowed the identification of C- and O-geranylated derivatives, and the protease (subtilisin and pepsin) inhibition performed on the O-geranylated compounds showed weak inhibition. Electrophoretic profiles indicated the presence of bands/spots among 56-58 kDa and pI 6-7, which are compatible with prenyltransferases. These findings show that P. crassinervium could be considered as a source of extracts with geranyltransferase activity to perform biotransformations on aromatic substrates. (C) 2010 Elsevier Ltd. All rights reserved. | |
dc.language | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation | Bioresource Technology | |
dc.relation | 5.807 | |
dc.relation | 2,029 | |
dc.rights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | Piper crassinervium | |
dc.subject | Piperaceae | |
dc.subject | Biotransformation | |
dc.subject | Geranylation | |
dc.title | Geranylation of benzoic acid derivatives by enzymatic extracts from Piper crassinervium (Piperaceae) | |
dc.type | Artigo | |