dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | Universidade Federal de São Carlos (UFSCar) | |
dc.date.accessioned | 2014-05-20T14:17:44Z | |
dc.date.accessioned | 2022-10-05T15:14:21Z | |
dc.date.available | 2014-05-20T14:17:44Z | |
dc.date.available | 2022-10-05T15:14:21Z | |
dc.date.created | 2014-05-20T14:17:44Z | |
dc.date.issued | 2010-12-15 | |
dc.identifier | Biochemical Engineering Journal. Lausanne: Elsevier B.V. Sa, v. 53, n. 1, p. 7-11, 2010. | |
dc.identifier | 1369-703X | |
dc.identifier | http://hdl.handle.net/11449/25318 | |
dc.identifier | 10.1016/j.bej.2009.05.006 | |
dc.identifier | WOS:000285898800003 | |
dc.identifier | 2478051365107560 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3898434 | |
dc.description.abstract | Amino acids are well metabolized by Streptomyces clavuligerus during the production of clavulanic acid using glycerol as main carbon and energy source. However, only a few amino acids such as arginine and ornithine are favorable for CA biosynthesis. The aim of this work was to optimize the glycerol:ornithine molar ratio in the feed medium containing only these compounds to maximize CA production in continuous cultivation. A minimum number of experiments were performed by means of a simple two-level full-factorial central composite design to investigate the combined effect of glycerol and ornithine feeding on the CA concentration during the intermittent and continuous process in shake-flasks. Statistical analysis of the experimental data using the response surface methodology showed that a glycerol-to-ornithine molar ratio of approximately 40:1 in the feed medium resulted in the highest CA concentration when fermentation was stopped. Under these optimized conditions, in bench-scale fermentor runs, the CA concentration reached more than double the concentration obtained in shake-flasks runs. (C) 2009 Elsevier B.V. All rights reserved. | |
dc.language | eng | |
dc.publisher | Elsevier B.V. Sa | |
dc.relation | Biochemical Engineering Journal | |
dc.relation | 3.226 | |
dc.rights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | Clavulanic acid | |
dc.subject | Submerged culture | |
dc.subject | Streptomyces clavuligerus | |
dc.subject | Continuous culture | |
dc.subject | Glycerol | |
dc.subject | Ornithine | |
dc.subject | Bioprocess design | |
dc.subject | Optimisation | |
dc.title | Optimisation of the glycerol-to-ornithine molar ratio in the feed medium for the continuous production of clavulanic acid by Streptomyces clavuligerus | |
dc.type | Artigo | |