dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:03:17Z
dc.date.accessioned2022-10-05T14:52:42Z
dc.date.available2014-05-20T14:03:17Z
dc.date.available2022-10-05T14:52:42Z
dc.date.created2014-05-20T14:03:17Z
dc.date.issued1999-01-01
dc.identifierCytobios. Cambridge: Faculty Press, v. 100, n. 394, p. 119-126, 1999.
dc.identifier0011-4529
dc.identifierhttp://hdl.handle.net/11449/22296
dc.identifierWOS:000085203300006
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3895930
dc.description.abstractElectron microscopy and immunolabelling with antiserum specific to cucumber mosaic virus coat protein were used to examine tobacco leaf cells infected by cucumber mosaic virus isolated from Catharanthus roseus (CMV-Cr). Crystalline and amorphous inclusions in the vacuoles were the most obvious cytological modifications seen. Immunogold labelling indicated that the crystalline inclusion was made up of virus particles and amorphous inclusions contained coat protein. Rows of CMV-Cr particles were found between membranes of dictyosomes, but membranous bodies and tonoplast-associated vesicles were not evident. Virus particles and/or free coat protein were easily detected in the cytoplasm by immunolabelling. No gold labelling was found within nuclei, chloroplasts and mitochondria.
dc.languageeng
dc.publisherFaculty Press
dc.relationCytobios
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcucumovirus
dc.subjectimmunolabelling
dc.subjectelectron microscopy
dc.subjectVinca rosea
dc.subjectcapsid protein
dc.titleSubcellular localization of the coat protein in tobacco cells infected by cucumber mosaic virus isolated from Catharanthus roseus
dc.typeArtigo


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