dc.contributor | Universidade de São Paulo (USP) | |
dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | Universidade Federal do Rio Grande do Norte (UFRN) | |
dc.contributor | Netherlands Red Cross | |
dc.contributor | Indoor Biotechnol Inc | |
dc.date.accessioned | 2014-05-20T13:56:02Z | |
dc.date.accessioned | 2022-10-05T14:36:03Z | |
dc.date.available | 2014-05-20T13:56:02Z | |
dc.date.available | 2022-10-05T14:36:03Z | |
dc.date.created | 2014-05-20T13:56:02Z | |
dc.date.issued | 2008-04-01 | |
dc.identifier | Journal of Allergy and Clinical Immunology. New York: Mosby-elsevier, v. 121, n. 4, p. 1040-1046, 2008. | |
dc.identifier | 0091-6749 | |
dc.identifier | http://hdl.handle.net/11449/20036 | |
dc.identifier | 10.1016/j.jaci.2007.12.1147 | |
dc.identifier | WOS:000254884000034 | |
dc.identifier | 2901888624506535 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3894003 | |
dc.description.abstract | Background: Evidence indicates that infection with Ascaris lumbricoides may promote development of allergy and asthma.Objective: To study the role of tropomyosin, a pan-allergen in invertebrates, in IgE responses to A lumbricoides.Methods: Recombinant A lumbricoides and Periplaneta americana tropomyosins were expressed in Pichia pastoris. Levels of IgE to tropomyosins from A lumbricoides and P americana were determined by chimeric ELISA in sera from 119 children living in a parasite-endemic area and 112 patients with cockroach allergy from the allergy clinics. Presence of tropomyosin in A lumbricoides larvae at L3 stage was evaluated by immunofluorescence using mAb IA6, directed against mite tropomyosin. Molecular modeling of P americana and A lumbricoides tropomyosins was performed by using the MODELLER program.Results: A lumbricoides tropomyosin showed 69% to 98% sequence identity to tropomyosins from other invertebrates. The predicted structure of A lumbricoides tropomyosin was similar to that of P americana tropomyosin and showed the characteristic coiled-coil structure. Strong correlation was found for IgE antibodies to tropomyosins from A lumbricoides and P americana in sera from children living in a parasite-endemic area and from patients with cockroach allergy. Larvae of A lumbricoides reacted strongly with mAb IA6.Conclusion: Tropomyosin induces IgE responses in A lumbricoides-infected children and in patients allergic to cockroach. | |
dc.language | eng | |
dc.publisher | Mosby-elsevier | |
dc.relation | Journal of Allergy and Clinical Immunology | |
dc.relation | 13.258 | |
dc.relation | 5,049 | |
dc.rights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | tropomyosin | |
dc.subject | Ascaris lumbricoides | |
dc.subject | periplaneta americana | |
dc.subject | IgE cross-reactivity | |
dc.subject | allergy | |
dc.subject | parasites | |
dc.subject | asthma | |
dc.title | Cross-reactive IgE antibody responses to tropomyosins from Ascaris lumbricoides and cockroach | |
dc.type | Artigo | |