dc.contributorUniversidade Estadual de Londrina (UEL)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:54:22Z
dc.date.accessioned2022-10-05T14:32:00Z
dc.date.available2014-05-20T13:54:22Z
dc.date.available2022-10-05T14:32:00Z
dc.date.created2014-05-20T13:54:22Z
dc.date.issued2007-02-01
dc.identifierToxicology In Vitro. Oxford: Pergamon-Elsevier B.V., v. 21, n. 1, p. 41-52, 2007.
dc.identifier0887-2333
dc.identifierhttp://hdl.handle.net/11449/19433
dc.identifier10.1016/j.tiv.2006.07.018
dc.identifierWOS:000244169500006
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3893538
dc.description.abstractA large number of functional foods, including those that contain P-glucan, have been shown to prevent the development of cancer and other chronic diseases. The aim of the present study was to elucidate its mechanism of action, as well as to understand its effects as an antigenotoxic, anticlastogenic agent, and to determine its capacity to preserve cell viability. The investigation was carried out in the CHO-k1 and CHO-xrs5 cell lines. The cytokinesis-blocked micronucleus assay indicated that the different doses of beta-glucan examined (5, 10, 20 and 40 mu g/ml) did not show clastogenic effects. In the CHO-k1 cell line, a chemopreventive effect could be observed in all the protocols tested: pre-treatment (% reduction of 35.0-57.3), simultaneous treatment (simple - 5 reduction of 19.7-55.6 and with pre-incubation - of 42.7-56.4) and post-treatment (% reduction of 17.9-37.6). This finding indicates mechanisms of action involving desmutagenesis and bio-antimutagenesis, albeit the latter having a lesser role. However, in the repair-deficient CHO-xrs5 cells, beta-glucan did not show a protective effect with post-treatment (% reduction of 2.96), thus supporting the involvement of bioantimutagenesis. The comet assay in CHO-k1 cells demonstrated that beta-glucan has neither a genotoxic nor an antigenotoxic effect. Cell viability tests indicated that beta-glucan preserves cell viability in both cell lines, preventing apoptotic events. These findings suggest that beta-glucan, when present in foods, could provide them with nutraceutical characteristics and act as a dietary supplement, or that P-glucan could be used in new drug development. (c) 2006 Elsevier Ltd. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationToxicology in Vitro
dc.relation3.105
dc.relation0,931
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectbeta-glucan
dc.subjectCHO-xrs5 cells
dc.subjectCHO-k1
dc.subjectcomet assay
dc.subjectcytokinesis-blocked micronucleus test
dc.titleProtective effect of beta-glucan extracted from Saccharomyces cerevisiae, against DNA damage and cytotoxicity in wild-type (k1) and repair-deficient (xrs5) CHO cells
dc.typeArtigo


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