dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorInstituto de Pesquisas Energéticas e Nucleares (IPEN)
dc.date.accessioned2014-05-20T13:51:19Z
dc.date.accessioned2022-10-05T14:25:03Z
dc.date.available2014-05-20T13:51:19Z
dc.date.available2022-10-05T14:25:03Z
dc.date.created2014-05-20T13:51:19Z
dc.date.issued2002-08-01
dc.identifierToxicon. Oxford: Pergamon-Elsevier B.V., v. 40, n. 8, p. 1101-1106, 2002.
dc.identifier0041-0101
dc.identifierhttp://hdl.handle.net/11449/18332
dc.identifier10.1016/S0041-0101(02)00103-4
dc.identifierWOS:000177655500003
dc.identifier9353490382598257
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3892761
dc.description.abstractThe ability of gamma radiation from Co-60 (2000 Gy) to attenuate the toxic effects of Bothrops jararacussu venom was investigated on mouse neuromuscular preparations in vitro. A comparative study between the effects of native and irradiated venoms was performed on both phrenic-diaphragm (PD) and extensor digitorum longus (EDL) preparations by means of myographic, biochemical and morphological techniques. Native venom (10 and 20 mug/ml) induced a concentration-dependent paralysis of both directly and indirectly evoked contractions on PD preparations. At 20 mug/ml, it also caused a pronounced myotoxic effect on the EDL muscle preparation that was characterized by an increase of creatine kinase release and by several morphological changes of this preparation. By contrast, irradiated venom, even at concentrations as high as 40 mug/ml, induced neither paralyzing nor myotoxic effects. It was concluded that Co-60 gamma radiation is able to abolish both the paralyzing and the myotoxic effects of B. jararacussu venom on the mouse neuromuscular junction. These findings support the hypothesis that gamma radiation could be an important toot to improve antisera production by reducing toxicity while preserving immunogenicity. (C) 2002 Elsevier B.V. Ltd. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationToxicon
dc.relation2.352
dc.relation0,692
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectgamma radiation
dc.subjectBothrops jararacussu venom
dc.subjectneuromuscular junction
dc.titleCo-60 gamma irradiation prevents Bothrops jararacussu venom neurotoxicity and myotoxicity in isolated mouse neuromuscular junction
dc.typeArtigo


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