dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:50:18Z
dc.date.accessioned2022-10-05T14:23:06Z
dc.date.available2014-05-20T13:50:18Z
dc.date.available2022-10-05T14:23:06Z
dc.date.created2014-05-20T13:50:18Z
dc.date.issued2008-01-01
dc.identifierGenetics and Molecular Biology. Sociedade Brasileira de Genética, v. 31, n. 1, p. 303-307, 2008.
dc.identifier1415-4757
dc.identifierhttp://hdl.handle.net/11449/17953
dc.identifier10.1590/S1415-47572008000200025
dc.identifierS1415-47572008000200025
dc.identifierWOS:000257238500025
dc.identifierS1415-47572008000200025.pdf
dc.identifier8858800699425352
dc.identifier0804793944846367
dc.identifier1926863917378909
dc.identifier0000-0003-4600-0367
dc.identifier0000-0003-3534-974X
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3892530
dc.description.abstractIn order to extend the genetic data on the Sciaenidae fish family, the present study had the purpose to characterize PCR-generated 5S rDNA repeats of twelve species of this group through PAGE (Polyacrylamide Gel Electrophoresis) analysis. The results showed the occurrence of at least two different 5S rDNA size classes in all the species. Moreover, 5S rDNA repeats of one of the studied species - Isopisthus parvipinnis - were cloned and subjected to nucleotide sequencing and Southern blot membrane hybridization analyses, which permitted to confirm the existence of two major 5S rDNA classes. Phylogenetic analysis based on the nucleotide sequences of different 5S rDNA repeats of I. parvipinnis lead to their separation into two major clusters. These results may reflect the high dynamism that rules the evolution rate of 5S rDNA repeats. The obtained data suggest that 5S rDNA can be useful in genetic analyses to identify species-specific markers and determine relationships among species of the Sciaenidae group.
dc.languageeng
dc.publisherSociedade Brasileira de Genética
dc.relationGenetics and Molecular Biology
dc.relation1.493
dc.relation0,638
dc.rightsAcesso aberto
dc.sourceSciELO
dc.subjectSciaenidae
dc.subjectfish
dc.subjectIsopisthus parvipinnis
dc.subject5S rDNA
dc.subjectmolecular markers
dc.title5S rDNA characterization in twelve Sciaenidae fish species (Teleostei, Perciformes): depicting gene diversity and molecular markers
dc.typeArtigo


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