Artigo
Evaluation of monocot and eudicot divergence using the sugarcane transcriptome(1[w])
Fecha
2004-03-01Registro en:
Plant Physiology. Rockville: Amer Soc Plant Biologists, v. 134, n. 3, p. 951-959, 2004.
0032-0889
10.1104/pp.103.033878
WOS:000220360400010
7179273060624761
0165348738208319
0000-0003-4524-954X
Autor
Universidade de São Paulo (USP)
Universidade Federal da Paraíba (UFPB)
Universidade Federal de São Carlos (UFSCar)
Universidade Estadual Paulista (Unesp)
Univ Mogi das Cruzes
Inst Agron Campinas
Inst Agronom Campinas
Ctr Tecnol Copersucar
Univ Ribeirao Preto
Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Estadual de Campinas (UNICAMP)
Resumen
Over 40,000 sugarcane (Saccharum officinarum) consensus sequences assembled from 237,954 expressed sequence tags were compared with the protein and DNA sequences from other angiosperms, including the genomes of Arabidopsis and rice (Oryza sativa). Approximately two-thirds of the sugarcane transcriptome have similar sequences in Arabidopsis. These sequences may represent a core set of proteins or protein domains that are conserved among monocots and eudicots and probably encode for essential angiosperm. functions. The remaining sequences represent putative monocot-specific genetic material, one-half of which were found only in sugarcane. These monocot-specific cDNAs represent either novelties or, in many cases, fast-evolving sequences that diverged substantially from their eudicot homologs. The wide comparative genome analysis presented here provides information on the evolutionary changes that underlie the divergence of monocots and eudicots. Our comparative analysis also led to the identification of several not yet annotated putative genes and possible gene loss events in Arabidopsis.