dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | Universidade de São Paulo (USP) | |
dc.contributor | Fac Associadas Sao Paola | |
dc.contributor | Univ Costa Rica | |
dc.date.accessioned | 2014-05-20T13:49:18Z | |
dc.date.accessioned | 2022-10-05T14:20:33Z | |
dc.date.available | 2014-05-20T13:49:18Z | |
dc.date.available | 2022-10-05T14:20:33Z | |
dc.date.created | 2014-05-20T13:49:18Z | |
dc.date.issued | 1999-06-15 | |
dc.identifier | Archives of Biochemistry and Biophysics. San Diego: Academic Press Inc., v. 366, n. 2, p. 177-182, 1999. | |
dc.identifier | 0003-9861 | |
dc.identifier | http://hdl.handle.net/11449/17558 | |
dc.identifier | 10.1006/abbi.1999.1210 | |
dc.identifier | WOS:000080904800001 | |
dc.identifier | 9162508978945887 | |
dc.identifier | 0000-0003-2460-1145 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3892229 | |
dc.description.abstract | Lys49-Phospholipase A(2) (Lys49-PLA(2)) homologues damage membranes by a Ca2+-independent mechanism which does not involve catalytic activity. With the aim of determining the structural basis for this novel activity, we have solved the crystal structure of myotoxin-II, a Lys49-PLA(2) isolated from the venom of Cerrophidion (Bothrops) godmani (godMT-II) at 2.8 Angstrom resolution by molecular replacement. The final model has been refined to a final crystallografic residual (R-factor) of 18.8% (R-free = 28.2%), with excellent stereochemistry. godMT-II is also monomeric in the crystalline state, and small-angle X-ray scattering results demonstrate that the protein is monomeric in solution under fisicochemical conditions similar to those used in the crystallographic studies. (C) 1999 Academic Press. | |
dc.language | eng | |
dc.publisher | Academic Press Inc. | |
dc.relation | Archives of Biochemistry and Biophysics | |
dc.relation | 3.118 | |
dc.relation | 1,350 | |
dc.rights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | snake venoms | |
dc.subject | Lys49-Phospholipase A(2) | |
dc.subject | myotoxins | |
dc.subject | Cerrophidion (Bothrops) godmani | |
dc.title | Crystal structure of myotoxin II, a monomeric Lys49-Phospholipase A(2) homologue isolated from the venom of Cerrophidion (Bothrops) godmani | |
dc.type | Artigo | |