dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:33:37Z
dc.date.accessioned2022-10-05T13:41:32Z
dc.date.available2014-05-20T13:33:37Z
dc.date.available2022-10-05T13:41:32Z
dc.date.created2014-05-20T13:33:37Z
dc.date.issued2008-07-01
dc.identifierBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 41, n. 7, p. 615-620, 2008.
dc.identifier0100-879X
dc.identifierhttp://hdl.handle.net/11449/11512
dc.identifier10.1590/S0100-879X2008000700011
dc.identifierS0100-879X2008000700011
dc.identifierWOS:000259326100011
dc.identifierS0100-879X2008000700011.pdf
dc.identifier5406518799128485
dc.identifier9418970103564137
dc.identifier8727897080522289
dc.identifier4125344753100454
dc.identifier0000-0003-1270-7372
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3887501
dc.description.abstractObesity is a complex multifactorial disorder that is often associated with cardiovascular diseases. Research on experimental models has suggested that cardiac dysfunction in obesity might be related to alterations in myocardial intracellular calcium (Ca2+) handling. However, information about the expression of Ca2+-related genes that lead to this abnormality is scarce. We evaluated the effects of obesity induced by a high-fat diet in the expression of Ca2+-related genes, focusing the L-type Ca2+ channel (Cacna1c), sarcolemmal Na+/Ca2+ exchanger (NCX), sarcoplasmic reticulum Ca2+ ATPase (SERCA2a), ryanodine receptor (RyR2), and phospholamban (PLB) mRNA in rat myocardium. Male 30-day-old Wistar rats were fed a standard (control) or high-fat diet (obese) for 15 weeks. Obesity was defined as increased percent of body fat in carcass. The mRNA expression of Ca2+-related genes in the left ventricle was measured by RT-PCR. Compared with control rats, the obese rats had increased percent of body fat, area under the curve for glucose, and leptin and insulin plasma concentrations. Obesity also caused an increase in the levels of SERCA2a, RyR2 and PLB mRNA (P < 0.05) but did not modify the mRNA levels of Cacna1c and NCX. These findings show that obesity induced by high-fat diet causes cardiac upregulation of Ca2+ transport_related genes in the sarcoplasmic reticulum.
dc.languageeng
dc.publisherAssociação Brasileira de Divulgação Científica (ABRADIC)
dc.relationBrazilian Journal of Medical and Biological Research
dc.relation1.492
dc.rightsAcesso aberto
dc.sourceSciELO
dc.subjectObesity
dc.subjectHigh-fat diet
dc.subjectRat heart
dc.subjectCa2+ cycling
dc.subjectmRNA
dc.subjectCa2+-related genes
dc.titleObesity induces upregulation of genes involved in myocardial Ca2+ handling
dc.typeArtigo


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