dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2014-05-20T13:25:38Z
dc.date.accessioned2022-10-05T13:17:09Z
dc.date.available2014-05-20T13:25:38Z
dc.date.available2022-10-05T13:17:09Z
dc.date.created2014-05-20T13:25:38Z
dc.date.issued2007-05-01
dc.identifierCanadian Journal of Physiology and Pharmacology. Ottawa: Natl Research Council Canada-n R C Research Press, v. 85, n. 5, p. 536-545, 2007.
dc.identifier0008-4212
dc.identifierhttp://hdl.handle.net/11449/8148
dc.identifier10.1139/Y07-037
dc.identifierWOS:000249010900008
dc.identifier1445259468526188
dc.identifier0000-0002-0970-4288
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3884843
dc.description.abstractAugmented glucose-stimulated insulin secretion (GSIS) is an adaptive mechanism exhibited by pancreatic islets from insulin-resistant animal models. Gap junction proteins have been proposed to contribute to islet function. As such, we investigated the expression of connexin 36 (Cx36), connexin 43 (Cx43), and the glucose transporter Glut2 at mRNA and protein levels in pancreatic islets of dexamethasone (DEX)-induced insulin-resistant rats. Study rats received daily injections of DEX (1 mg/kg body mass, i.p.) for 5 days, whereas control rats (CTL) received saline solution. DEX rats exhibited peripheral insulin resistance, as indicated by the significant postabsorptive insulin levels and by the constant rate for glucose disappearance (K-ITT). GSIS was significantly higher in DEX islets (1.8-fold in 16.7 mmol/L glucose vs. CTL, p < 0.05). A significant increase of 2.25-fold in islet area was observed in DEX vs. CTL islets (p < 0.05). Cx36 mRNA expression was significantly augmented, Cx43 diminished, and Glut2 mRNA was unaltered in islets of DEX vs. CTL (p < 0.05). Cx36 protein expression was 1.6-fold higher than that of CTL islets (p < 0.05). Glut2 protein expression was unaltered and Cx43 was not detected at the protein level. We conclude that DEX-induced insulin resistance is accompanied by increased GSIS and this may be associated with increase of Cx36 protein expression.
dc.languageeng
dc.publisherNatl Research Council Canada-n R C Research Press
dc.relationCanadian Journal of Physiology and Pharmacology
dc.relation2.210
dc.relation0,724
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectconnexins
dc.subjectglucocorticoids
dc.subjectinsulin resistance
dc.subjectglucose-stimulated insulin secretion
dc.subjectpancreatic islets
dc.titleDexamethasone-induced insulin resistance is associated with increased connexin 36 mRNA and protein expression in pancreatic rat islets
dc.typeArtigo


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