Cheap assays of malate, glycerol and ethanol in fermenting must and food samples

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:24:30Z
dc.date.accessioned2022-10-05T13:13:53Z
dc.date.available2014-05-20T13:24:30Z
dc.date.available2022-10-05T13:13:53Z
dc.date.created2014-05-20T13:24:30Z
dc.date.issued2003-01-01
dc.identifierEuromedlab 2003: Proceedings of the 15th Ifcc-fescc European Congress of Clinical Chemistry and Laboratory Medicine. 40128 Bologna: Medimond S R L, p. 383-386, 2003.
dc.identifierhttp://hdl.handle.net/11449/7614
dc.identifierWOS:000184180200070
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3884447
dc.description.abstractA preparation, enriched with malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), glycerol -3- P dehydrogenase (GPDH) and glycerol kinase (GK), was obtained from dry baker's yeast. This preparation was used to assay glycerol, ethanol and malate measuring the variations in absorbance (NADH formation) at 340 nm. Good degrees of recoveries were obtained when glycerol was added to red wine and fermenting sugar-cane juice and when L-malate was added to commercial apple juice samples. Good results were also obtained when ethanol was assayed in fermented sugar-cane juice and wine samples, using both the partially purified preparation obtained from dry yeast and a purified commercial alcohol dehydrogenase.
dc.languageeng
dc.publisherMedimond S R L
dc.relationEuromedlab 2003: Proceedings of the 15th Ifcc-fescc European Congress of Clinical Chemistry and Laboratory Medicine
dc.rightsAcesso aberto
dc.sourceWeb of Science
dc.subjectdry baker's yeast
dc.subjectglycerol
dc.subjectethanol
dc.subjectmalate assay
dc.titleCheap assays of malate, glycerol and ethanol in fermenting must and food samples
dc.typeTrabalho apresentado em evento


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