dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:23:57Z
dc.date.accessioned2022-10-05T13:12:22Z
dc.date.available2014-05-20T13:23:57Z
dc.date.available2022-10-05T13:12:22Z
dc.date.created2014-05-20T13:23:57Z
dc.date.issued2012-08-01
dc.identifierFems Yeast Research. Hoboken: Wiley-blackwell, v. 12, n. 5, p. 557-570, 2012.
dc.identifier1567-1356
dc.identifierhttp://hdl.handle.net/11449/7322
dc.identifier10.1111/j.1567-1364.2012.00806.x
dc.identifierWOS:000306189600006
dc.identifier0000-0002-8059-0826
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3884254
dc.description.abstractParacoccidioidomycosis is a systemic mycosis caused by the dimorphic fungus Paracoccidioides brasiliensis. Understanding the interactions between P.similar to brasiliensis and the host tissue depends on the study of the different steps of the process of colonization, especially adhesion, in which the pathogen recognizes ligands on the surface of host cells. This study aimed to verify the role of enolase in the host cellfungus interaction and the ability of enolase to bind to extracellular matrix components, to determine its subcellular localization, and to study the P.similar to brasiliensis enolase amino acid sequence. The data revealed that fibronectin is the major ligand of enolase. Evaluation of the location of enolase at an ultrastructural level revealed that it is distributed in various cellular compartments, but at a high level in the cell wall. The analysis of the amino acid sequence revealed an internal plasminogen-binding motif (254FYKADEKKY262), which is conserved in most organisms and described as an important interaction site of the enolase with the host cell surface. This suggests that enolase performs additional functions related to the glycolytic pathway and also plays a role of adhesion in P.similar to brasiliensis. Therefore, this study increases the knowledge about the characteristics of enolase and its influence on the binding process of P.similar to brasiliensis.
dc.languageeng
dc.publisherWiley-Blackwell
dc.relationFEMS Yeast Research
dc.relation2.609
dc.relation1,308
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectParacoccidioides brasiliensis
dc.subjectenolase
dc.subjectadhesion
dc.subjectextracellular matrix
dc.subjectimmunogold
dc.titleSurface-expressed enolase contributes to the adhesion of Paracoccidioides brasiliensis to host cells
dc.typeArtigo


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