dc.contributorUniv Oxford
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:12:46Z
dc.date.accessioned2022-10-05T12:25:20Z
dc.date.available2014-05-20T13:12:46Z
dc.date.available2022-10-05T12:25:20Z
dc.date.created2014-05-20T13:12:46Z
dc.date.issued2006-09-01
dc.identifierMolecular Microbiology. Oxford: Blackwell Publishing, v. 61, n. 5, p. 1352-1361, 2006.
dc.identifier0950-382X
dc.identifierhttp://hdl.handle.net/11449/701
dc.identifier10.1111/j.1365-2958.2006.05316.x
dc.identifierWOS:000239701400021
dc.identifierWOS000239701400021.pdf
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3879144
dc.description.abstractRegulation of chromosome inheritance is essential to ensure proper transmission of genetic information. To accomplish accurate genome segregation, cells organize their chromosomes and actively separate them prior to cytokinesis. In Bacillus subtilis the Spo0J protein is required for accurate chromosome segregation and it regulates the developmental switch from vegetative growth to sporulation. Spo0J is a DNA-binding protein that recognizes at least eight identified parS sites located near the origin of replication. As judged by fluorescence microscopy, Spo0J forms discrete foci associated with the oriC region of the chromosome throughout the cell cycle. In an attempt to determine the mechanisms utilized by Spo0J to facilitate productive chromosome segregation, we have investigated the DNA binding activity of Spo0J. In vivo we find Spo0J associates with several kilobases of DNA flanking its specific binding sites (parS) through a parS-dependent nucleation event that promotes lateral spreading of Spo0J along the chromosome. Using purified components we find that Spo0J has the ability to coat non-specific DNA substrates. These 'Spo0J domains' provide large structures near oriC that could potentially demark, organize or localize the origin region of the chromosome.
dc.languageeng
dc.publisherBlackwell Publishing
dc.relationMolecular Microbiology
dc.relation3.816
dc.rightsAcesso aberto
dc.sourceWeb of Science
dc.titleThe bacterial chromosome segregation protein Spo0J spreads along DNA from parS nucleation sites
dc.typeArtigo


Este ítem pertenece a la siguiente institución