Implementação de um sistema de eletroforese capilar com detecção de fluorescência induzida por laser
Development of a laser induced fluorescence detection scheme for capillary electrophoresis
dc.creator | Santos, Marcia R. | |
dc.creator | Tavares, Marina F. M. | |
dc.creator | Rubim, Joel C. | |
dc.date.accessioned | 2017-12-07T04:32:51Z | |
dc.date.accessioned | 2022-10-04T13:46:04Z | |
dc.date.available | 2017-12-07T04:32:51Z | |
dc.date.available | 2022-10-04T13:46:04Z | |
dc.date.created | 2017-12-07T04:32:51Z | |
dc.date.issued | 2000 | |
dc.identifier | Quím. Nova,v.23,n.5,p.585-589,2000 | |
dc.identifier | http://repositorio.unb.br/handle/10482/25599 | |
dc.identifier | https://dx.doi.org/10.1590/S0100-40422000000500003 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3851057 | |
dc.description.abstract | A capillary electrophoresis system using laser induced fluorescence detection is described. A Raman system equipped with a microscope has been used to focus the laser beam on the capillary giving a lateral resolution of 1.5 mm. The fluorescence signal of the analyte (ZnPcTS - tetrasulfonated zinc-phthalocyanine) was collected by the microscope objectives and analysed by a monochromator with confocal characteristics equipped with a CCD detector. Electropherograms obtained with this system were compared to those obtained on a commercial instrument, showing that the described system presents a lower detection limit and better resolution. | |
dc.language | pt | |
dc.publisher | Sociedade Brasileira de Química | |
dc.rights | Acesso Aberto | |
dc.title | Implementação de um sistema de eletroforese capilar com detecção de fluorescência induzida por laser | |
dc.title | Development of a laser induced fluorescence detection scheme for capillary electrophoresis | |
dc.type | Artículos de revistas |