dc.contributorDenise Aparecida Andrade de Oliveira
dc.contributorhttp://lattes.cnpq.br/5853314951388292
dc.contributorÂngela Maria Quintão Lana
dc.contributorLívia Loiola dos Santos
dc.contributorLilian Vianna Teixeira
dc.contributorEduardo Geraldo Alves Coelho
dc.creatorDanielle Cunha Cardoso
dc.date.accessioned2022-07-05T15:30:00Z
dc.date.accessioned2022-10-04T00:04:53Z
dc.date.available2022-07-05T15:30:00Z
dc.date.available2022-10-04T00:04:53Z
dc.date.created2022-07-05T15:30:00Z
dc.date.issued2020-02-28
dc.identifierhttp://hdl.handle.net/1843/42924
dc.identifier0000-0003-2145-5891
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3831372
dc.description.abstractGenomic variant calling, in order to identify new single nucleotide variants (SNVs) and insertions/deletions (InDELs), potentially associated with the characteristics of rusticity and resistance to arid climate in Nordestino horse breed, based in whole genome sequencing was the strategy used in the present study to understand the adaptive mechanisms of the breed to semiarid climate of Brazilian northeastern. These variations are possibly associated with the resistance exhibited by the breed, through the highly specialized racial pattern for the performance of activities that require strength and endurance, without any damage to its perpetuation. Complete genome sequencing of a typical representative of the breed was carried out, this being the first whole genome sequencing of a Brazilian naturalized equine breed, as a comparative genomics strategy for variants calling with the most recent reference genome equine update (Ensemble EquCab 3.0). Tools based on algorithms for variants calling identified 1,598,210 SNVs and 138,139 InDels (Freebayes software) and 88,838 SNVs and 25,232 InDELs (GATK software). Variants were classified as to the type, location and impact effect and the genomic regions were anoted. High and moderate impact variants were selected to functional enrichment analysis and for population validation in 60 animals from the states of Bahia, Pernambuco and Piaui. High-impact SNVs occurred exclusively in GTPase genes. Olfactory Receptor (OR) genes family containing moderate-impact SNVs had high representativeness in the functional analysis. All 10 loci tested, containing breed-specific SNVs, presented polymorphic alleles in the three populations, with high genotypic frequencies of heterozygous and homozygous with allelic polymorphism. The genotypic frequencies for 8 loci were significantly different between the three populations, suggesting allelic fixation and genetic diversity. The presence of SNVs in OR genes, which are related to adaptive mechanisms, represents important genetic information to understand the adaptation of the breed to the semiarid region of northeastern Brazil and to associate them with the peculiar phenotype exhibited by that breed.
dc.publisherUniversidade Federal de Minas Gerais
dc.publisherBrasil
dc.publisherVET - DEPARTAMENTO DE ZOOTECNIA
dc.publisherPrograma de Pós-Graduação em Zootecnia
dc.publisherUFMG
dc.rightsAcesso Aberto
dc.subjectCavalo
dc.subjectGenética
dc.subjectvalidação populacional
dc.subjectZootecnia
dc.titleSequenciamento de genoma completo do cavalo Nordestino para identificação de variantes genômicas (SNVs e INDELs) e validação em subpopulações remanescentes
dc.typeTese


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