Efeitos indiretos da tolerância oral a um componente da dieta (Zeína) no reparo de feridas na pele de camundongos

Abstract

Early in the twentieth century it was observed that certain materials associated with the antigen, such as aluminum hydroxide (Al (OH) 3) served as adjuvant greatly increased the production of antibodies. On the other hand, also in the early twentieth century it was observed that mice that had been fed with feed containing corn not respond immunologically to protein of maize, such as zein, as did other specimens which had not been fed corn. Today we know that the absorption of intact proteins or partially digested by intestinal mucosa is a common occurrence after meals. These proteins interfere with the activity of the immune system and a result of these interferences is usual oral tolerance, a phenomenon consisting in reducing immune responses to proteins previously contacted orally. The first reports on the influence of dietary proteins on the immune activity were made with plant and animal proteins but most studies of oral tolerance have been made using animal-derived proteins such as ovalbumin (OVA), bovine gamablobulina (BGG) and keyhole limpet hemocyanin (KLH). Since these proteins are not part of animals usual diet of mice, they are introduced in the feed or diluted in water for oral tolerance induction. Thus we know that animals ingest OVA before immunization with OVA + Al (OH) 3 OVA antibodies to form less than that do not ingest OVA. However the ingestion of OVA immunization does not interfere with other proteins, except that during the immunization with this second protein is also given one immunization with the protein tolerated. This last observation has been studied by our group a few years show that immunization of tolerant animals with antigen in adjuvant tolerated inhibits immune responses to unrelated antigens and also inhibits inflammatory reactions, such as those triggered by carrageenan. This phenomenon triggered by injection of tolerated antigen has been termed "indirect effects of oral tolerance." We show that the indirect effects of injection of OVA in OVA-tolerant mice inhibit inflammation after skin lesions in mice resulting in less scarring. Evaluate the indirect effects Oral tolerance on the cutaneous lesions and they also occur with a protein present in the usual diet of mice. We zein, forming part of protein composition of the feed of the mice and model incision and excision of the skin. The indirect effects of parenteral immunization with a tolerated protein (zein from corn) reduces the inflammatory infiltrate in the area of incision and excision on days 5, 7 and 40 respectively, improves the repair and reduces the scar formed by deposition of an extracellular matrix structure more similar to normal skin. The secondary immunization with a protein tolerated without adjuvant and concomitant damage to the skin of mice, did not reduce the inflammatory infiltrate and the area of granulation tissue histologically analyzing the skin at days after injury.