Dissertação
Citotoxicidade, genotoxicidade e expressão gênica em células-tronco mesenquimais expostas a materiais endodônticos
Fecha
2021-07-30Autor
Patrícia Yanne de Oliveira
Institución
Resumen
On the market, we found a wide variety of endodontics cements available for clinical
use, but several studies show divergences of opinion regarding the biological
behavior of these different materials. This work aimed to investigate cell viability and
metabolism, an expression of genes involved in cell plasticity and cell differentiation
in stem cell cultures recovered from human dental pulp (hDPSCs) when in contact
with four endodontic cements (Endofill, MTA, Pulp Canal Sealer, Sealer 26) routinely
used in endodontic clinic. It also aimed, through a systematic review, to analyze the
biocompatibility of endodontic materials on dental stem cells. For this, the viability
and metabolism of hDPSCs, when it comes into contact with capillaries that included
or not cements, was assessed by MTT assay (24 and 48 hours) and exclusion of
trypan blue assay (48 hours). Cellular plasticity, with the presence of capillaries
containing or not sealers, was evaluated by the genetic expression of the markers
CD34, CD45, Nestin, CD105, Nanog and OCT-4 by PCR. Finally, cell differentiation
from endodontics sealers was verified by the expression of the RUNX2, ALP,
OC/BGLAP and DMP1 genes by RT-PCR. The data were analyzed using the
ANOVA test with Bonferroni correction (p<0.05). We note that Pulp Canal Sealer and
Endofill sealers decrease cell viability and cellular metabolism when compared to
control after 48 hours (p<0.001). MTA and Sealer 26 did not interfere with cell
viability in the two evaluation periods (p>0.05). hDPSCs, when grown in the presence
of MTA and Sealer 26, express the Nestin, CD105, NANOG and OCT-4 markers, and
do not express CD34 and CD45. In turn, MTA and Sealer 26 interfered in the gene
expression of DMP1, OC/BGLAP and RUNX2 in relation to the control group
(p<0.05), but did not find a significant difference in relation to the ALP gene
expression (p>0.05). Therefore, MTA and Sealer 26 demonstrate good biological
compatibility when in the presence of hDPSCs. The systematic review showed that
almost all materials have good compatibility when in contact with stem cells, being
able to be used in clinical practice