Caracterização molecular de herpesvírus bovinos por análise da região codificadora da glicoproteína G

dc.contributorEdel Figueiredo Barbosa
dc.contributorNelson Rodrigo da Silva Martins
dc.contributorClarice Weis Arns
dc.contributorMaurilio Andrade Rocha
dc.creatorGuilherme Domingos Romano Oliveira
dc.date.accessioned2019-08-14T21:23:53Z
dc.date.accessioned2022-10-03T23:07:21Z
dc.date.available2019-08-14T21:23:53Z
dc.date.available2022-10-03T23:07:21Z
dc.date.created2019-08-14T21:23:53Z
dc.date.issued2006-04-10
dc.identifierhttp://hdl.handle.net/1843/SSLA-7TZNNY
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3817110
dc.description.abstractA semi-nested PCR was standardized to amplify the gG coding region of Bovine Herpesvirus (BpHV). This PCR was able to identify standard sample of BoHV-1.1 (Colorado ATCC 864), BoHV-1.2 (K22) and BoHV-5 (EVI-88). This test was also efficient in indentifying infection of cell cultures inoculated with material from semen, nasal swabs and central nervous system (CNS). The efficiency of the test was evaluated by restriction endonuclease assay with XhoI and by the amplicons sequencing. The genic region used showed to be efficient in identifying BoHV-1 and BoHV-5 in samples. For the regions and conditions used, the Heteroduplex Mobility Assay (HMA) showed to be unsatisfactory for differentiating subgroups. A co-infection of Bovine Syncytial Virus (BRSV) and BoHV-5 was detected in cows of a dairy farm. This report has alsoshowed BoHV-5 envolved in respiratory disorder in adult animals
dc.publisherUniversidade Federal de Minas Gerais
dc.publisherUFMG
dc.rightsAcesso Aberto
dc.subjectBoHV 1
dc.subjectCo-infection
dc.subjectBoHV 12
dc.subjectBRSV
dc.subjectSequencing
dc.subjectBoHV 5
dc.subjectPCR
dc.titleCaracterização molecular de herpesvírus bovinos por análise da região codificadora da glicoproteína G
dc.typeDissertação de Mestrado


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