Dissertação
RT-PCR quantitativa para detecção de DENV em larvas de Aedes SP
Fecha
2011-02-18Autor
Flávia Viana Ferreira
Institución
Resumen
Dengue virus 1 to 4 (family Flaviviridae, genus Flavivirus) (DENV-1, 2, 3, 4), are
responsible for an estimated 50 million cases annually. Infections caused by
any of the four serotypes can cause an acute febrile illness and a more serious,
often fatal, characterized by hemorrhage and shock syndrome. The virus is
mainly transmitted by Aedes aegypti and Aedes albopictus, when DENVinfected, transmit the virus to the host at the time of blood meal. Mosquitoes
may acquire the virus by biting an infected person, male to female (venereal
transmission) and through the progeny. Vertical transmission occurs only when
the virus multiplies in the ovariole of the female and infected eggs. Because
there is no specific therapy, the most effective method of disease control is
direct combat the vector. In addition, there are few studies addressing vector
competence, which is the ability to infect mosquitoes, and to keep replicating
the virus spread. Why this study was to evaluate the viral load of DENV pools of
Aedes sp collected in Belo Horizonte-MG. The evaluation was done by
amplification of DNA from the 5'UTR region of DENV and the control of
glutamine synthetase gene from Aedes sp by qPCR. To develop this technique
was tested a mixture composed of reagents that detect and amplify DENV
allowed in pool of larvae and even a single larva. To determine the number of
copies of DENV by larvae, additional tests should still be made. Thus, the
results of this study indicate that it was possible to detect, through a more
sensitive technique, DENV on Aedes sp, and with prospects for a quantification
of the numbers of copies. Studies using this methodology will be important to
assess vector competence, stating, on the numbers of infected larvae, the risk
of dengue transmission in a given area and thus assist in the control and
prevention of dengue in Brazil.