dc.contributorMarcos Bryan Heinemann
dc.contributorAurora Maria Guimaraes Gouvea
dc.contributorRomulo Cerqueira Leite
dc.contributorClaudio Jose Borela Espeschit
dc.contributorMarcelo Fernandes Camargos
dc.contributorZelia Ines Portela Lobato
dc.contributorAlice Andrioli Pinheiro
dc.creatorJuliano Cezar Minardi da Cruz
dc.date.accessioned2019-08-13T02:07:05Z
dc.date.accessioned2022-10-03T22:33:08Z
dc.date.available2019-08-13T02:07:05Z
dc.date.available2022-10-03T22:33:08Z
dc.date.created2019-08-13T02:07:05Z
dc.date.issued2009-02-27
dc.identifierhttp://hdl.handle.net/1843/SSLA-83KNSV
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3805114
dc.description.abstractCaprine arthritis-encephalitis (CAE) is a multisystemic viral syndrome in goats caused by small ruminant lentivirus (CAEV). The control measures prescribed for CAEV control are based on the indentification of infected animals through a suitable serological test. The aim of this work was to improve the CAE control measures through the association of indirect (agar gel immunodiffusion-AGID) and direct (nested-PCR) assays to CAEV diagnosis. AGID was performed at birth before colostrum, at day of infection and throughout 12 months pos infection. We studied the viral shedding in a group of nine experimental infected males over a year by following the proviral load by nested-PCR in semen and blood. During the experimental period (EP) no clinical signs of CAE were observed. Throughout the EP one animal (1/9) remained AGID-seronegative to CAEV. When the semen samples were submitted to PCR, that same AGID-seronegative animal showed positive results. Delayed seroconversion appears to be a feature of CAEV infection wich may have direct implications for eradication programs and epidemiologic studies that rely on serologic methods to detect infected goats. Our results suggest that the bucks show intermittent shedding of proviral DNA into ejaculated semen. No significant relationship was found between the presence of proviral CAEV DNA in blood and the detection of proviral CAEV DNA in ejaculates. We conclude that the classical management practices recommended for CAEV control are insufficient in CAEV eradication programs and that PCR may be a useful tool for decreasing the risk of breeding AGID false negative animals
dc.publisherUniversidade Federal de Minas Gerais
dc.publisherUFMG
dc.rightsAcesso Aberto
dc.subjectlentivírus
dc.subjectCaprino
dc.subjectPCR
dc.subjectsêmen
dc.subjectLVPR
dc.titleMonitoramento sorológico e da presença do DNA pró-viral do lentivirus caprino (CAEV) no sangue e semen de reprodutores infectados
dc.typeTese de Doutorado


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