dc.creator | Massip Copiz, María Macarena | |
dc.creator | Valdivieso, Ángel Gabriel | |
dc.creator | Clauzure, Mariángeles | |
dc.creator | Mori, Consuelo | |
dc.creator | Asensio, Cristian | |
dc.creator | Aguilar, María Á. | |
dc.creator | Santa Coloma, Tomás Antonio | |
dc.date.accessioned | 2022-05-27T13:30:26Z | |
dc.date.available | 2022-05-27T13:30:26Z | |
dc.date.created | 2022-05-27T13:30:26Z | |
dc.date.issued | 2021 | |
dc.identifier | Massip Copiz, M. M. et al. Epidermal growth factor receptor activity upregulates lactate dehydrogenase a expression, lactate dehydrogenase activity, and lactate secretion in cultured ib3-1 cystic fibrosis lung epithelial cells [en línea]. Postprint de artículo publicado en Biochemistry and Cell Biology. 2021, 99 (4). doi: 10.1139/bcb-2020-0522. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/14036 | |
dc.identifier | 0829-8211 (impreso) | |
dc.identifier | 1208-6002 (on line) | |
dc.identifier | https://repositorio.uca.edu.ar/handle/123456789/14036 | |
dc.identifier | 10.1139/bcb-2020-0522 | |
dc.identifier | 33481676 | |
dc.description.abstract | Abstract: Cystic fibrosis (CF) is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. It has been postulated that reduced HCO3− transport through CFTR may lead to a decreased airway surface liquid pH. In contrast, others have reported no changes in the extracellular pH (pHe). We have recently reported that in carcinoma Caco-2/pRS26 cells (transfected with short hairpin RNA for CFTR) or CF lung epithelial IB3-1 cells, the mutation in CFTR decreased mitochondrial complex I activity and increased lactic acid production, owing to an autocrine IL-1β loop. The secreted lactate accounted for the reduced pHe, because oxamate fully restored the pHe. These effects were attributed to the IL-1β autocrine loop and the downstream signaling kinases c-Src and JNK. Here we show that the pHe of IB3-1 cells can be restored to normal values (∼7.4) by incubation with the epidermal growth factor receptor (EGFR, HER1, ErbB1) inhibitors AG1478 and PD168393. PD168393 fully restored the pHe values of IB3-1 cells, suggesting that the reduced pHe is mainly due to increased EGFR activity and lactate. Also, in IB3-1 cells, lactate dehydrogenase A mRNA, protein expression, and activity are downregulated when EGFR is inhibited. Thus, a constitutive EGFR activation seems to be responsible for the reduced pHe in IB3-1 cells. | |
dc.language | eng | |
dc.rights | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.rights | Acceso abierto | |
dc.source | Postprint de artículo publicado en Biochemistry and Cell Biology. 2021, 99 (4) | |
dc.subject | FIBROSIS QUISTICA | |
dc.subject | GENES | |
dc.subject | SISTEMA RESPIRATORIO | |
dc.subject | CELULAS | |
dc.subject | LACTATO DESHIDROGENASA | |
dc.title | Epidermal growth factor receptor activity upregulates lactate dehydrogenase a expression, lactate dehydrogenase activity, and lactate secretion in cultured ib3-1 cystic fibrosis lung epithelial cells | |
dc.type | Artículos de revistas | |