dc.creatorMassip Copiz, María Macarena
dc.creatorValdivieso, Ángel Gabriel
dc.creatorClauzure, Mariángeles
dc.creatorMori, Consuelo
dc.creatorAsensio, Cristian
dc.creatorAguilar, María Á.
dc.creatorSanta Coloma, Tomás Antonio
dc.date.accessioned2022-05-27T13:30:26Z
dc.date.available2022-05-27T13:30:26Z
dc.date.created2022-05-27T13:30:26Z
dc.date.issued2021
dc.identifierMassip Copiz, M. M. et al. Epidermal growth factor receptor activity upregulates lactate dehydrogenase a expression, lactate dehydrogenase activity, and lactate secretion in cultured ib3-1 cystic fibrosis lung epithelial cells [en línea]. Postprint de artículo publicado en Biochemistry and Cell Biology. 2021, 99 (4). doi: 10.1139/bcb-2020-0522. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/14036
dc.identifier0829-8211 (impreso)
dc.identifier1208-6002 (on line)
dc.identifierhttps://repositorio.uca.edu.ar/handle/123456789/14036
dc.identifier10.1139/bcb-2020-0522
dc.identifier33481676
dc.description.abstractAbstract: Cystic fibrosis (CF) is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. It has been postulated that reduced HCO3− transport through CFTR may lead to a decreased airway surface liquid pH. In contrast, others have reported no changes in the extracellular pH (pHe). We have recently reported that in carcinoma Caco-2/pRS26 cells (transfected with short hairpin RNA for CFTR) or CF lung epithelial IB3-1 cells, the mutation in CFTR decreased mitochondrial complex I activity and increased lactic acid production, owing to an autocrine IL-1β loop. The secreted lactate accounted for the reduced pHe, because oxamate fully restored the pHe. These effects were attributed to the IL-1β autocrine loop and the downstream signaling kinases c-Src and JNK. Here we show that the pHe of IB3-1 cells can be restored to normal values (∼7.4) by incubation with the epidermal growth factor receptor (EGFR, HER1, ErbB1) inhibitors AG1478 and PD168393. PD168393 fully restored the pHe values of IB3-1 cells, suggesting that the reduced pHe is mainly due to increased EGFR activity and lactate. Also, in IB3-1 cells, lactate dehydrogenase A mRNA, protein expression, and activity are downregulated when EGFR is inhibited. Thus, a constitutive EGFR activation seems to be responsible for the reduced pHe in IB3-1 cells.
dc.languageeng
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rightsAcceso abierto
dc.sourcePostprint de artículo publicado en Biochemistry and Cell Biology. 2021, 99 (4)
dc.subjectFIBROSIS QUISTICA
dc.subjectGENES
dc.subjectSISTEMA RESPIRATORIO
dc.subjectCELULAS
dc.subjectLACTATO DESHIDROGENASA
dc.titleEpidermal growth factor receptor activity upregulates lactate dehydrogenase a expression, lactate dehydrogenase activity, and lactate secretion in cultured ib3-1 cystic fibrosis lung epithelial cells
dc.typeArtículos de revistas


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