Artículos de revistas
RNA-seq analysis reveals TRPC genes to impact an unexpected number of metabolic and regulatory pathways
Fecha
2020Registro en:
2045-2322 (online)
10.1038/s41598-020-61177-x
Autor
Formoso, Karina
Susperreguy, Sebastián
Freichel, Marc
Birnbaumer, Lutz
Institución
Resumen
Abstract: The seven-member transient receptor potential canonical genes (TRPC1-7) encode cation channels
linked to several human diseases. There is little understanding of the participation of each TRPC in each
pathology, considering functional redundancy. Also, most of the inhibitors available are not specific.
Thus, we developed mice that lack all of the TRPCs and performed a transcriptome analysis in eight
tissues. The aim of this research was to address the impact of the absence of all TRPC channels on gene
expression. We obtained a total of 4305 differentially expressed genes (DEGs) in at least one tissue
where spleen showed the highest number of DEGs (1371). Just 21 genes were modified in all the tissues.
Performing a pathway enrichment analysis, we found that many important signaling pathways were
modified in more than one tissue, including PI3K (phosphatidylinositol 3-kinase/protein kinase-B)
signaling pathway, cytokine-cytokine receptor interaction, extracellular matrix (ECM)-receptor
interaction and circadian rhythms. We describe for the first time the changes at the transcriptome level
due to the lack of all TRPC proteins in a mouse model and provide a starting point to understand the
function of TRPC channels and their possible roles in pathologies.