Artículos de revistas
In vitro study of the proliferation and growth of human fetal osteoblasts on Mg and Si co-substituted tricalcium phosphate ceramics
Fecha
2017-04-20Autor
García-Páez, Ismael H.
de Aza, Antonio H.
Parra, Juan
Baudin, Carmen
Martín, M. Rocío
Pena, Pilar
Institución
Resumen
The objective of this work was to study the feasibility of the solid state sintering, a conventional ceramic processing method, to obtain Mg and Si co-substituted tricalcium phosphate bioceramics and composites containing diopside. A series of new Ca3(PO4)2 based ceramics has been prepared from attrition milled mixtures of synthetic Ca3(PO4)2 and CaMg(SiO3)2 powders, isostatically pressed and sintered at 1250–1300°C. Materials containing 0, 1, and 5 wt % of CaMg(SiO3)2 were constituted by β + α - Ca3(PO4)2 solid solutions while the material containing 60 wt % of CaMg(SiO3)2 was a constituted by β- Ca3(PO4)2 and CaMg(SiO3)2. The biological responses of the developed ceramics were studied in vitro using human fetal osteoblast cultures. Culture times ranged from 1 to 21 days. The new family of materials promotes the adhesion and proliferation of human osteoblasts cultured onto their surface forming a monolayer and showing a normal morphology. The results of the MTT and Alamar Blue assays showed that the soluble components extracted from the Mg/Si- co-substituted Ca3(PO4)2 and the Ca3(PO4)2-CaMg(SiO3)2 composite were noncytotoxic. The specimens with diopside exhibited a better in vitro behavior which is attributed to the release of Si and Mg ions to the culture medium, enhancing the activity of cells. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2266–2275, 2017.