Evaluación in – silico de la estructura y función de la proteína hipotética B7FQK1 de phaeodactylum tricornutum

dc.contributorSánchez Calderón, Juan David
dc.creatorNavarro Gómez, Sirlhey
dc.creatorSuárez Gómez, Ana Milena
dc.date.accessioned2020-01-22T20:48:52Z
dc.date.available2020-01-22T20:48:52Z
dc.date.created2020-01-22T20:48:52Z
dc.identifierhttps://hdl.handle.net/10901/17781
dc.description.abstractPhaeodactylum tricornutum es una diatomea marina objeto de estudio durante los últimos años gracias a sus propiedades biológicas y su potencial biotecnológico. A partir de P. tricornutum se pueden obtener distintos componentes de alto valor como nutracéuticos, biocombustibles, cosméticos, productos farmacéuticos, etc. Esta microalga se encuentra dentro de las principales especies productoras de PUFAs (EPA y DHA), importantes en la industria farmacéutica y alimentaria debido a sus efectos positivos en la salud humana. P. tricornutum posee un genoma de aproximadamente 27. 4 megabases (Mb) y se estima que contiene 10, 402 genes. No obstante, existen regiones génicas con funcionalidad desconocida, lo que genera la necesidad de llevar a cabo análisis bioinformáticos que faciliten la comprensión del flujo de información desde los genes a las estructuras moleculares. Es por esto que, se buscó predecir computacionalmente la estructura de la proteína hipotética B7FQK1 de Phaeodactylum tricornutum y comprobar la función descrita, relacionada con la biosíntesis de ácidos grasos. La investigación se desarrolló en cuatro fases, la primera consistió en la evaluación in – silico de la estructura primaria, utilizando servidores y algoritmos como TMHMM, ConSurf, PROSITE, Pfam y BLAST. Posteriormente, se analizaron las características físico – químicas y perfiles de la secuencia de aminoácidos con las herramientas EXPASY – PROTPARAM y ProtScale respectivamente. En la tercera fase se predijo la estructura secundaria a partir de los resultados obtenidos de los servidores NPS@ y PSIPRED. Por último, se obtuvo la construcción del modelo 3D de la proteína mediante el servidor I – TASSER y se validó con la herramienta STRUCTURE ASSESSMENT de SWISS – MODEL. Se identificó un dominio FA_desaturasa 2 directamente relacionado con la función predicha. Con base en la evaluación computacional, se obtuvo la estructura secundaria y el modelo 3D, este último con un C – score de 1. 75 que indica un modelo de buena calidad. La predicción estructural y funcional de la proteína hipotética B7FQK1 permite profundizar en los conocimientos de las propiedades biológicas de la microalga y contribuye en la optimización de los procesos biotecnológicos.
dc.description.abstractPhaeodactylum tricornutum is a marine diatom that has been studied in recent years due to its biological properties and its biotechnological potential. From P. tricornutum different high value components can be obtained such as nutraceuticals, biofuels, cosmetics, pharmaceutical products, etc. This microalga is among the main producer species of PUFAs (EPA and DHA) important in the pharmaceutical and food industry due to its positive effects on human health. P. tricornutum has its sequenced genome, it has approximately 27.4 megabases (Mb) and it is estimated that it contains 10,402 genes. However, there are gene regions with unknown functionality, which generates the need to carry out bioinformatics analysis that facilitates the understanding of the flow of information from genes to molecular structures. That is why, we sought computationally to predict the structure of the hypothetical protein B7FQK1 of Phaeodactylum tricornutum and verify the function described, related to the biosynthesis of fatty acids. The research was developed in four phases, the first consisted in the in-silico evaluation of the primary structure, using servers and algorithms such as TMHMM, ConSurf, PROSITE, Pfam and BLAST. Subsequently, the physicochemical characteristics and profiles of the amino acids sequence were analyzed with the EXPASY - PROTPARAM and ProtScale tools respectively. In the third phase the secondary structure was predicted from the results obtained from the NPS @ and PSIPRED servers. Finally, the construction of the 3D model of the protein was obtained through the I - TASSER server and validated with the STRUCTURE ASSESSMENT of SWISS - MODEL tool. A FA_desaturase 2 domain directly related to the predicted function was identified. Based on the computational evaluation, the secondary structure and the 3D model were obtained, the latter with a C - score of 1.75 indicating a good quality model. The structural and functional prediction of the hypothetical protein B7FQK1 allows deepening the knowledge of the biological properties of the microalga and contributes in the optimization of biotechnological processes.
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dc.rightshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/
dc.rightsAtribución-NoComercial-SinDerivadas 2.5 Colombia
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subjectÁcidos grasos
dc.subjectEnergía biomasica
dc.subjectMicroalgas
dc.titleEvaluación in – silico de la estructura y función de la proteína hipotética B7FQK1 de phaeodactylum tricornutum


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