dc.contributor | Ropero Vega, Jose Luis | |
dc.creator | Rodríguez Caicedo, Juliana Paola | |
dc.date.accessioned | 2022-08-02T20:43:12Z | |
dc.date.available | 2022-08-02T20:43:12Z | |
dc.date.created | 2022-08-02T20:43:12Z | |
dc.date.issued | 2022-08-02 | |
dc.identifier | Rodríguez Caicedo, J.P. (2022). Detección electroquímica de Escherichia coli O157:H7 en agua utilizando biosensores peptídicos modificados [Tesis de Maestría]. Universidad Santo Tomás. Bucaramanga, Colombia. | |
dc.identifier | http://hdl.handle.net/11634/46337 | |
dc.identifier | reponame:Repositorio Institucional Universidad Santo Tomás | |
dc.identifier | instname:Universidad Santo Tomás | |
dc.identifier | repourl:https://repository.usta.edu.co | |
dc.description.abstract | The contamination generated by the presence of microorganisms such as Escherichia coli O157:H7 represents an environmental and social problem, due to the serious diseases presented by this pathogen. The timely detection of this microorganism presents some disadvantages with respect to traditional techniques, therefore, it is necessary to develop new technologies for the detection of this microorganism in water with shorter detection times, therefore, in this work it is presented an alternative for the detection of Escherichia coli O157:H7 through the use of electrochemical biosensors based on PEPTIR peptides.
The determination of the analytical characteristics of the peptide-based electrochemical biosensor PEPTIR was carried out by immobilizing PEPTIR 1 and PEPETIR 2 at different concentrations of 5, 10, 50 and 100 nM, seeking to guarantee the immobilization and detection of Escherichia coli O157: H7. For this, the analytical characteristics of the electrochemical biosensors were evaluated using electrochemical techniques such as cyclic voltammetry (CV), square wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS), thus evaluating the effect of the modifications made on the working electrode. of the electrochemical biosensor. Obtaining as a result the optimal concentrations of PEPTIR 1.0 and PEPTIR 2.0, improving the detection limits and quantification limit at different concentrations of microorganism 10, 20, 50, 100, 300 CFU/mL. On the other hand, the selectivity of the peptide-based biosensor for PEPTIR 1.0 and PEPTIR 2.0 against Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus was evaluated, resulting in a high selectivity with respect to other microorganisms. | |
dc.language | spa | |
dc.publisher | Universidad Santo Tomás | |
dc.publisher | Maestría Ciencias y Tecnologías Ambientales | |
dc.publisher | Facultad de Química Ambiental | |
dc.relation | Alcaraz, M. R., Fabiano, S. N., & Cámara, M. S. (2013). Desarrollo de un biosensor amperométrico de tirosinasa para la determinación de contenido fenólico total en aguas superficiales. | |
dc.relation | Alhadrami, H. A. (2018). Biosensors: Classifications, medical applications, and future prospective. Biotechnology and Applied Biochemistry. https://doi.org/10.1002/bab.1621 | |
dc.relation | Altug, H., Oh, S. H., Maier, S. A., & Homola, J. (2022). Advances and applications of nanophotonic biosensors. Nature Nanotechnology. https://doi.org/10.1038/s41565-021-01045-5 | |
dc.relation | Angel, K. S. O., Sánchez-evangelista, G., Carmona-navarrete, I., Galicia-sánchez, M. C., Gómez-luna, A., & Islas-arrollo, S. J. (2018). Péptidos antimicrobianos , una alternativa prometedora para el tratamiento de enfermedades infecciosas. 681–688. https://doi.org/10.24875/GMM.18003445 | |
dc.relation | Brosel-Oliu, S., Ferreira, R., Uria, N., Abramova, N., Gargallo, R., Muñoz-Pascual, F.-X., & Bratov, A. (2017). Novel impedimetric aptasensor for label-free detection of Escherichia coli O157:H7. https://doi.org/10.1016/j.snb.2017.09.121 | |
dc.relation | Cesewski, E., & Johnson, B. N. (2020a). Biosensors and Bioelectronics Electrochemical biosensors for pathogen detection. Biosensors and Bioelectronics, 159(April), 112214. https://doi.org/10.1016/j.bios.2020.112214 | |
dc.relation | Cesewski, E., & Johnson, B. N. (2020b). Electrochemical biosensors for pathogen detection. Biosensors and Bioelectronics. https://doi.org/10.1016/j.bios.2020.112214 | |
dc.relation | Chávez, M. (2017). Caracterización de Monocapas Autoensambladas de Tioles sobre Superficies de Oro. | |
dc.relation | Cho, I. H., Kim, D. H., & Park, S. (2020). Electrochemical biosensors: Perspective on functional nanomaterials for on-site analysis. Biomaterials Research. https://doi.org/10.1186/s40824-019-0181-y | |
dc.relation | González, V. (2015). Biosensores electroquímicos basados en nanomateriales y en materiales magnéticos para la determinación de analitos de interés bioquímico. | |
dc.relation | Gupta, R., Raza, N., Bhardwaj, S. K., Vikrant, K., Kim, K. H., & Bhardwaj, N. (2021). Advances in nanomaterial-based electrochemical biosensors for the detection of microbial toxins, pathogenic bacteria in food matrices. Journal of Hazardous Materials, 401, 123379. https://doi.org/10.1016/j.jhazmat.2020.123379 | |
dc.relation | Kaur, H., Bhosale, A., & Shrivastav, S. (2018). Biosensors: Classification, Fundamental Characterization and New Trends: A Review. International Journal of Health Sciences & Research (Www.Ijhsr.Org). www.ijhsr.org | |
dc.relation | Kulagina, N. V., Lassman, M. E., Ligler, F. S., & Taitt, C. R. (2005). Antimicrobial peptides for detection of bacteria in biosensor assays. Analytical Chemistry. https://doi.org/10.1021/ac050639r | |
dc.relation | López, L. S. (2021). Estudio de factores de virulencia en Escherichia coli. | |
dc.relation | Magar, H. S., Hassan, R. Y. A., & Mulchandani, A. (2021). Electrochemical impedance spectroscopy (Eis): Principles, construction, and biosensing applications. Sensors. https://doi.org/10.3390/s21196578 | |
dc.relation | Mirceski, V., Skrzypek, S., & Stojanov, L. (2018). Square-wave voltammetry. ChemTexts, 4(4), 1–14. https://doi.org/10.1007/s40828-018-0073-0 | |
dc.relation | Pandey, A., Gurbuz, Y., Ozguz, V., Niazi, J. H., & Qureshi, A. (2017). Biosensors and Bioelectronics Graphene-interfaced electrical biosensor for label-free and sensitive detection of foodborne pathogenic E . coli O157 : H7. Biosensors and Bioelectronic, 91(October 2016), 225–231. https://doi.org/10.1016/j.bios.2016.12.041 | |
dc.relation | Redondo, J. F. (2021). Evaluación de péptidos análogos a la proteína Tir Como Moléculas de Reconocimiento en Biosensores Electroquímicos para la Detección de Escherichia Coli O157:H7 en Matrices Acuosas. | |
dc.relation | Ren, S., Zhang, X., Li, Z., Jian, X., Zhao, J., & Yan, Y. (2021). Development of a pulse ‑ induced electrochemical biosensor based on gluconamide for Gram ‑ negative bacteria detection. Microchimica Acta. https://doi.org/10.1007/s00604-021-05073-9 | |
dc.relation | Roldán, C. (2019). Monitoreo de la formación de complejos coacervados y emulsiones O/W con el uso de espectroscopía de impedancia eléctrica y voltametría cíclica. | |
dc.relation | Ropero-vega, J. L., Redondo-ortega, J. F., Galvis-curubo, Y. J., Rond, P., & Marcela, J. (2021). on Electrochemical Biosensors for the Detection of E . coli O157 : H7 in an Aqueous Matrix. | |
dc.relation | Rosado-Porto, D., Bonivento-Calvo, J., Salcedo-Mendoza, S., Molina-Castillo, A., Maestre-Serrano, R., & García, A. D. C. (2021). Determinación de E. coli biotipo 1 y E. coli O157:H7 en canal de carne bovina en plantas de beneficio del departamento del Atlántico (Colombia). Revista de Investigaciones Veterinarias Del Perú. https://doi.org/10.15381/rivep.v32i3.18476 | |
dc.relation | Royano, S. (2020). Biosensores electroquímicos para la determinación de pesticidas en aguas. | |
dc.relation | Sánchez, V. H. (2016). Desarrollo de biosensores para la detección de hidrocarburos aromáticos en aguas marinas Programa. | |
dc.relation | Sandoval, G., & Herrera, E. (2018). Lipase, Phospholipase, and Esterase Biosensors (Review). 1835. | |
dc.relation | Zhong, M., Yang, L., Yang, H., Cheng, C., Deng, W., Tan, Y., & Xie, Q. (2018). An electrochemical immunobiosensor for ultrasensitive detection of Escherichia coli O157:H7 using CdS quantum dots-encapsulated metal-organic frameworks as signal-amplifying tags. Biosensors and Bioelectronic. https://doi.org/10.1016/j.bios.2018.11.001 | |
dc.rights | http://creativecommons.org/publicdomain/zero/1.0/ | |
dc.rights | Abierto (Texto Completo) | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.rights | http://purl.org/coar/access_right/c_abf2 | |
dc.rights | CC0 1.0 Universal | |
dc.title | Detección electroquímica de Escherichia coli O157:H7 en agua utilizando biosensores peptídicos modificados | |