bachelor thesis
Perfil químico y biológico de los aceites esenciales de Cymbopogon nardus y Cymbopogon martinii
Fecha
2020-04-03Registro en:
Céspedes Navarro,M.G.(2020).Perfil químico y biológico de los aceites esenciales de Cymbopogon nardus y Cymbopogon martinii [Tesis de pregrado]. Universidad Santo Tomás, Bucaramanga, Colombia
reponame:Repositorio Institucional Universidad Santo Tomás
instname:Universidad Santo Tomás
Autor
Céspedes Navarro, Mabel Gricelda
Institución
Resumen
The chromatographic profiles (reconstructed streams) of the oils evaluated were obtained by gas chromatography coupled to mass spectrometry. The main major compounds of essential oils according to the percentages of relative areas are: citronelal 19.4%, geraniol 18.1% and citronellol 14.9% corresponding to C. nardus, on the other hand, the compounds: geraniol 64 % and 14.8% geranyl acetate were found in greater proportion in the C.martinii essential oil.
The antimicrobial activity of the essential oils was evaluated by the plate microdilution method in order to establish the minimum inhibitory concentration (MIC) against Porphyromonas gingivalis and Enterococcus faecalis. In the case of P. gingivalis, both oils evaluated showed antimicrobial activity with a MIC of 250-500 µg / mL and a CBM of 500 µg / mL. On the contrary, none of the essential oils presented antimicrobial activity against Enterococcus faecalis, bearing in mind that no inhibition of bacterial growth was evident.
The phytotoxicity of essential oils was determined by EC50, using bioindicators such as cabbage seeds (Brassica oleracea), tomato (Lycopersicon esculentum) and onion bulbs (Allium cepa). It was obtained that the C. nardus essential oil generated a 65% root elongation inhibition at an EC50 of 418.54 ppm in Lycopersicon esculentum seed while for the embryo it did not show an inhibitory effect EC50> 1000 ppm. Likewise, this oil did not show any inhibitory effect against the Brassica oleraceae seed.
In contrast, the C. martinii essential oil reported a 65% inhibition in root elongation for the Lycopersicon esculentum seed an EC50 of 154 ppm and for Brassica oleracea a 55% inhibition at an EC50 of 778.9 ppm. Also, the AE presented a 67% inhibition for embryo growth in Lycopersicon esculentum at an EC50 of 407 ppm.
On the other hand, the genotoxic effect of the essential oils in bulbs of Allium cepa was evaluated. Different chromosomal aberrations were identified in the essential oils evaluated, identified as: bridges in anaphase, binucleated cells, stray chromosomes in anaphase and telophase. Likewise, an EC50 of 712 ppm was identified for the C. martinii essential oil and an EC50 of 3.90 ppm for C. nardus.