dc.contributor | https://orcid.org/0000-0003-4149-6623 | |
dc.contributor | https://scholar.google.es/citations?user=1Iz8bfEAAAAJ&hl=en | |
dc.contributor | https://scholar.google.com/citations?user=i-_R070AAAAJ&hl=es | |
dc.contributor | https://scholar.google.es/citations?user=qj7XoWUAAAAJ&hl=es | |
dc.contributor | http://scienti.colciencias.gov.co:8081/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000553778 | |
dc.contributor | https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001202367 | |
dc.contributor | https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000254410 | |
dc.creator | Leal Pinto, Sandra Milena. | |
dc.creator | Herrera Sandoval, Laura Viviana. | |
dc.creator | Vargas, Leonor Yamile. | |
dc.date.accessioned | 2020-05-20T15:45:28Z | |
dc.date.accessioned | 2022-09-28T13:32:39Z | |
dc.date.available | 2020-05-20T15:45:28Z | |
dc.date.available | 2022-09-28T13:32:39Z | |
dc.date.created | 2020-05-20T15:45:28Z | |
dc.date.issued | 2020-05-19 | |
dc.identifier | Leal Pinto, S. M., Herrera Sandoval, L. V., & Vargas, L. Y. (2019). In vitro susceptibility of Microsporum spp. and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives. Mycoses, 62(1), 41–50. https://doi.org/10.1111/myc.12844 | |
dc.identifier | http://hdl.handle.net/11634/23339 | |
dc.identifier | https://doi.org/10.1111/myc.12844 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3639972 | |
dc.description.abstract | Background: Microsporum s
p
p are keratinophilic dermatophytes that mainly invade
the stratum corneum of the skin and hair causing clinical symptoms associated with
tinea. Its treatment has several limitations and the search for new active molecules is
necessary. Objetive: To evaluate the antifungal and cytotoxic potential of Eugenia
caryophyllus essential oil (EO), eugenol, isoeugenol and methylisoeugenol against
Microsporum canis, M. gypseum and Vero cells. Methods: The EO was extracted by
conventional heating assisted hydrodistillation
, the eugenol obtained commercially
and the derivatives through Williamson synthesis. Minimal Inhibitory Concentration
(MICs), minimum fungicidal concentration, inhibition of radial mycelial growth and
germination inhibition were used to evaluate the antifungal activity. In addition,
a
colorimetric test was conducte
d to evaluate cytotoxic activity. Results: MIC and MFC
values for all compounds were 62.5 to 500
μg/ml for both of the species of
Microsporum evaluated
.
Also, concentrations of 300
μg/ml of the compounds
inhibited 100% of M. canis mycelium. The inhibition of germination was observed
after 6 hours of treatment (11.86 ±3.46
-85.31 ±0 %). No cytotoxicity was observed in
Vero cells (CC50
> 105
μg/ml), whereas terbinafin
e showed CC50 31.00±0.61
μg/ml.
Conclusions: Our study indicates an interesting bioactivity of isoeugenol and
methylisoeugenol
against M. canis, M. gypseum and mammalian cells. | |
dc.relation | Gómez E, Crespo V, Martínez L. Dermatofitosis. Piel. 2016;31:546 -559. | |
dc.relation | Zhan P, Liu W. The Changing Face of Dermatophytic Infections Worldwide. Mycopathologia . 2017;182(1 -2):77 -86. | |
dc.relation | Mejía MA, Santa C, Cadavid M, et al. Estudio etiológico y epidemiológico de las micosis cutáneas en un laboratorio de referencia – Antioquia – Colombia. CES Medicina. 2013; 27(1):7 -19 . | |
dc.relation | Bonifaz A. Micología Básica Médica. 5th ed. McGrawHill:108 -158 | |
dc.relation | Marchese A, Barbieri R, Coppo E, et al. Antimicrobial activity of eugenol and essential oils containing eugenol: A mechanistic viewpoint. Crit Rev Microbiol. 2017;43(6):668 -689. | |
dc.relation | Leem HH, Kim EO, Seo MJ, Choi SW. Anti -oxidant and anti -inflamatory activities of eugenol and its derivatives from clove (Eugenia caryophyllata Thunb.). J Korean Soc Food Sci Nutr. 2011 ; 40:1361 –1370. | |
dc.relation | Medeiros A, Lima EO, Leite de Souza E, Formiga MF, Nogueira V, Medeiros IA . Inhibitory effect of b -pinene, a - pinene and eugenol on the growth of potential infectious endocarditis causing Gram -positive bacteria. Rev Bras Cienc Farm . 2007;43(1):121 –127 | |
dc.relation | Upadhyay A, Arsi K, Wagle BR, et al. Trans -Cinnamaldehyde, Carvacrol, and Eugenol Reduce Campylobacter jejuni Colonization Factors and Expression of Virulence Genes in Vitro. Front Microbiol. 2017;25:713 doi: 10.3389/fmicb.2017.00713. | |
dc.relation | Yogalakshmi B, Viswanathan P, Anuradha CV. Investigation of anti - oxidant, anti -in ammatory and DNA -protective properties of eugenol in thioacetamide -induced liver injury in rats. Toxicology . 2010;268(3):204 –212. | |
dc.relation | De Oliveira F, Moura J, de Oliveira E. Investigation on mechanism of antifungal activity of eugenol against Trichophyton rubrum. Med Mycol. 2013;51(5):507 –513. 14. Gupta A, Tu L. Dermatophytes: Diagnosis and treatment. J Am Acad Dermatol. 2006;54(6):1050 - 1055. | |
dc.relation | Pinto E, Vale -Silva L, Cavaleiro C, Salgueiro L. Antifungal activity of the clove essential oil from Syzygium aromaticum on Candida, Aspergillus and dermatophyte species. J Med Microbiol. 2009;58(11):1454 -62. | |
dc.relation | Escobar M, Rodríguez K , Ruiz S, Pierdant M, Cerda E, Pozos A. Eugenol Toxicity in Human Dental Pulp Fibroblasts of Primary Teeth. J Clin Pediatr Dent . 2016;40(4):312 -318. | |
dc.relation | Nenoff P, Krüger C, Shaller J, Ginter -Hanselmayer G, Shulte - Beerbühl R, Tietz HJ. Mycology – an update Part 2: Dermatomycoses: Clinical picture and diagnostics. J Dtsch Dermatol Ges. 2014;12(9):749 -77. | |
dc.relation | Silva KVS, Lima MIO, Cardoso GN, Santos AS, Silva GS, Pereira FO. Inibitory effects of linalool on fungal pathogenicity of clinical isolates of Microsporum canis and Microsporum gypseum. Mycoses. 2016;60(6): 387 -393. | |
dc.relation | Gupta A, Tu L. Dermatophytes: Diagnosis and treatment. J Am Acad Dermatol. 2006;54(6):1050 -1055. | |
dc.relation | Zuluagaa A, Cáceres D, Arango K, de Bedouta C, Cano L. Epidemiología de la tinea capitis: 19 años de experiencia en un laboratorio clínico especializado en Colombia. Infectio. 2016; 20(4):225 -230 | |
dc.relation | Kaul S, Yadav S, Dogra S. Treatment of dermatophytosis in elderly, children, and pregnant women. Indian Dermatol Online J. 2017;8(5):310 -318. | |
dc.relation | Ginter -Hanselmayer G, Gupta A. Itraconazole in the Treatment of Tinea Capitis Caused by Microsporum canis: Experience in a Large Cohort. Pediatr Dermato. 2004;21(4): 499 –502. | |
dc.relation | Moriello K, Coyner K, Susan Paterson S, Mignon B. Diagnosis and treatment of dermatophytosis in dogs and cats. Vet Dermatol. 2017;28(3):266 –268. | |
dc.relation | Chaieb K, Hajlaoui H, Zmantar T, et al. The Chemical Composition and Biological Activity of Clove Essential Oil, Eugenia caryophyllata (Syzigium aromaticum L. Myrtaceae): A Short Review. Phytother Res. 2007; 21(6):501 –506. | |
dc.relation | Gülçin I. Antioxidant activity of eugenol: a structure –activity relation - ship study. J Med Food. 2011;14(9):975 – 985. | |
dc.relation | Han X, Parker LT. Anti -inflammatory activity of clove (Eugenia caryophyllata) essential oil in human dermal fibroblasts. Pharm Biol. 2017;55(1):1619 -1622. | |
dc.relation | Mahakittikun V, Soonthornchareonnon N, Foongladda S, Boitano JJ, Wangapai T, Ninsanit P. A preliminary study of the acaricidal activity of clove oil, Eugenia caryophyllus. Asian Pac J Allergy Immunol. 2014;32(1):46 -52. | |
dc.relation | Seixas FJ, da Franca Rodrigues KA, Guerra de Oliveira R, et al. Synthesis and In Vitro Anti Leishmania amazonensis Biological Screening of Morita -Baylis -Hillman Adducts Prepared from Eugenol, Thymol and Carvacrol. Molecules 2016;21(11):1483 doi:10.3390/molecules21111483. | |
dc.relation | Cooping LG, Duke SO. Natural product that have been used commercially as crop protection agents. Pest Manag Sci . 2006;63(6):6303 -6307. | |
dc.relation | Lee SJ, Han JI, Lee GS, et al. Antifungal effect of eugenol and nerolidol against Microsporum gypseum in a guinea pig model. Biol Pharm Bull. 2007;30(1):184 -188. | |
dc.relation | Rana IS, Rana AS, Rajak RC. Evaluation of antifungal activity in essential oil of the Syzygium aromaticum (L.) by extraction, purification and analysis of its main component eugenol. Braz J Microbiol. 2011; 42(4):269 -1277. | |
dc.relation | Gayoso CW, Lima EO, Oliveira VT, et al. Sensitivity of fungi isolated from onichomicosis to Eugenia caryophyllata essential oil and eugenol. Fitoterapia. 2005;76(2):247 –249. | |
dc.relation | De Paula SB, Bartelli TF, Di Raimo V, et al. Effect of Eugenol on Cell Surface Hydrophobicity, Adhesion, and Biofilm of Candida tropicalis and Candida dubliniensis Isolated from Oral Cavity of HIV -Infected Patients. Evidence -Based Complementary and Alternative Medicine. 2014; doi: 10.1155/2014/505204. | |
dc.relation | Pavela R. Acude, synergistic and antagonistic effect of some aromatic compound on the Spodoptera littoralis Boisd. (Lep. Noctuidae) larvae. Ind Crops Prod. 2014;60: 247 -258. | |
dc.relation | Rossi PG, Bao L, Luciani A, et al. (E) -Methylisoeugenol and Elemicin: Antibacterial Components of Daucus carota L. Essential Oil against Campylobacter jejuni. J Agric Food Chem. 2007;55(18): 7332−7336 | |
dc.relation | Baldo A, Monod M, Mathy A, et al. Mechanisms of skin adherence and invasion by dermatophytes. Mycoses. 2011; 55(3):218 -223. | |
dc.relation | Baldo A, Tabart J, Vermout S, et al. Secreted subtilisins of Microsporum canis are involved in adherence of arthroconidia to feline corneocytes. J Med Microbiol. 2008; 57(9):1152 -1156 | |
dc.relation | Binh LT, Beaufay C, Trong ND, et al. In Vitro Anti -Leishmanial Activity of Essential Oils Extracted from Vietnamese Plants. Molecules. 2017; 22(7):1071. doi: 10.3390/molecules22071071. | |
dc.relation | Prashar A, Locke IC, Evans CS. Cytotoxicity of clove (Syzygium aromaticum) oil and its major components to human skin cells. Cell Prolif. 2006;39(4):241 -248. | |
dc.relation | FDA. US Food Drug Administration. Guidance for Industry Concerns Related to the use of Clove Oil as an Anesthetic for Fish, Guideline No 150. S. Department of Health and Human Services Food and Drug Administration Center for Veterinary Medicine.https://www.fda.gov/downloads/animalveterinary/guidancecomplianceenfor cement/guidanceforindustry/ucm052520.pdf accessed october 2017. | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/2.5/co/ | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/2.5/co/ | |
dc.rights | Atribución-NoComercial-SinDerivadas 2.5 Colombia | |
dc.rights | Atribución-NoComercial-SinDerivadas 2.5 Colombia | |
dc.title | In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives | |
dc.type | Generación de Nuevo Conocimiento: Artículos publicados en revistas especializadas - Electrónicos | |