Extracción del principio activo de pastillas comerciales de Losartán Potásico y Clorhidrato de Metformina y el estudio de su terapia combinatoria con Doxorrubicina

dc.contributorJiménez Díaz, Elizabeth
dc.contributorUribe Ardila, Jesús Alfredo
dc.contributorMiscione, Gian Pietro
dc.contributorGrupo de Investigación en Bioquímica Aplicada
dc.creatorGalindo León, Laura Daniela
dc.date.accessioned2022-08-01T15:03:32Z
dc.date.available2022-08-01T15:03:32Z
dc.date.created2022-08-01T15:03:32Z
dc.date.issued2022-07-27
dc.identifierhttp://hdl.handle.net/1992/59449
dc.identifierinstname:Universidad de los Andes
dc.identifierreponame:Repositorio Institucional Séneca
dc.identifierrepourl:https://repositorio.uniandes.edu.co/
dc.description.abstractEl cáncer se ha convertido en la segunda causa de muerte a nivel mundial, responsable de casi 10 millones de muertes en 2020. Por ello, a lo largo de los años hemos buscado desarrollar nuevas terapias que puedan llegar a ser más efectivas y con la mínima cantidad de efectos adversos en el paciente. Una opción es el uso de la terapia combinatoria (TC), en la que se pueden utilizar combinaciones de fármacos con acción antitumoral para conseguir una mayor eficacia con dosis más bajas, reduciendo los efectos adversos. Dentro de estas nuevas terapias, se ha encontrado que diferentes fármacos que se utilizan para el tratamiento de otras enfermedades como la diabetes o la hipertensión han mostrado actividad sobre células de diferentes tipos de cáncer. Además, dado que estos medicamentos ya están aprobados por las agencias reguladoras, los costos de investigación más bajos y otras ventajas podrían hacer que este tipo de terapias sea más accesible. Con todo esto en mente, el presente proyecto tiene como objetivo diseñar un protocolo de extracción para obtener los principios activos de los medicamentos Losartán Potásico y Clorhidrato de Metformina a partir de pastillas comerciales y caracterizar lo que sucede a nivel molecular estudiando los mecanismos de acción de estos medicamentos en combinación con la quimioterapia doxorrubicina mediante técnicas como la citometría de flujo y los ensayos de Western blot para comprender los determinantes su interacción en línea celular de cáncer colorrectal HCT116.
dc.languagespa
dc.publisherUniversidad de los Andes
dc.publisherMaestría en Química
dc.publisherFacultad de Ciencias
dc.publisherDepartamento de Química
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dc.titleExtracción del principio activo de pastillas comerciales de Losartán Potásico y Clorhidrato de Metformina y el estudio de su terapia combinatoria con Doxorrubicina
dc.typeTrabajo de grado - Maestría


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