dc.contributor | Universidad EAFIT. Departamento de Ciencias | |
dc.contributor | Ciencias Biológicas y Bioprocesos (CIBIOP) | |
dc.creator | Trujillo-Roldan, Mauricio A. | |
dc.creator | Valdez-Cruz, Norma A. | |
dc.creator | Gonzalez-Monterrubio, Cesar F. | |
dc.creator | Acevedo-Sanchez, Eduardo V. | |
dc.creator | Martinez-Salinas, Carlos | |
dc.creator | Garcia-Cabrera, Ramses I. | |
dc.creator | Gamboa-Suasnavart, Ramses A. | |
dc.creator | Marin-Palacio, Luz D. | |
dc.creator | Villegas, Jesus | |
dc.creator | Blancas-Cabrera, Abel | |
dc.date.accessioned | 2021-03-23T20:08:54Z | |
dc.date.accessioned | 2022-09-23T21:31:44Z | |
dc.date.available | 2021-03-23T20:08:54Z | |
dc.date.available | 2022-09-23T21:31:44Z | |
dc.date.created | 2021-03-23T20:08:54Z | |
dc.date.issued | 2013-11-01 | |
dc.identifier | 01757598 | |
dc.identifier | 14320614 | |
dc.identifier | WOS;000351609200004 | |
dc.identifier | SCOPUS;2-s2.0-84925331497 | |
dc.identifier | http://hdl.handle.net/10784/26789 | |
dc.identifier | 10.1007/s00253-013-5199-9 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3530752 | |
dc.description.abstract | Azospirillum brasilense has industrial significance as a growth promoter in plants of commercial interest. However, there is no report in the literature disclosing a liquid product produced in pilot-scale bioreactors and is able to be stored at room temperature for more than 2 years. The aim of this work was to scale up a process from a shake flask to a 10-L lab-scale and 1,000-L pilot-scale bioreactor for the production of plant growth-promoting bacterium A. brasilense for a liquid inoculant formulation. Furthermore, this work aimed to determine the shelf life of the liquid formulation stored at room temperature and to increase maize crops yield in greenhouses. Under a constant oxygen mass transfer coefficient (K L a), a fermentation process was successfully scaled up from shake flasks to 10- and 1,000-L bioreactors. A concentration ranging from 3.5 to 7.5 × 108 CFU/mL was obtained in shake flasks and bioreactors, and after 2 years stored at room temperature, the liquid formulation showed one order of magnitude decrease. Applications of the cultured bacteria in maize yields resulted in increases of up to 95 % in corncobs and 70 % in aboveground biomass. © 2013 Springer-Verlag Berlin Heidelberg. | |
dc.language | eng | |
dc.publisher | SPRINGER | |
dc.relation | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84888435786&doi=10.1007%2fs00253-013-5199-9&partnerID=40&md5=b83617d3b2eb42cb5ccd64bfbf7a0acd | |
dc.rights | https://v2.sherpa.ac.uk/id/publication/issn/0175-7598 | |
dc.source | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | |
dc.title | Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation | |
dc.type | article | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.type | publishedVersion | |