Quantification of SARS-CoV-2 antibodies with eight commercially available immunoassays
Autor
Weidner, Lisa
Gänsdorfer, Simon
Unterweger, Stephan
Weseslindtner, Lukas
Drexler, Camilla
Farcet, Maria
Witt, Volker
Schistal, Elisabeth
Schlenke, Peter
Kreil, Thomas R.
Jungbauer, Christof
Institución
Resumen
Since the emergence of SARS-CoV-2 numerous antibody assays have become available, demonstrating different
performance characteristics. This study focused on a quantitative correlation between different commercial
assays and a neutralization test (NT). Comparative data is needed as a basis for the production of convalescent
plasma and potential interpretations COVID-19 immunity.
Sera of 100 SARS-CoV-2 convalescent plasma donors were collected and SARS-CoV-2 antibodies were
characterized using three different IgG-ELISAs (EUROIMMUN IgG and NCP-IgG ELISA, Wantai ELISA), two CLIA
(Elecsys, LIAISON) and two lateral flow tests (MEDsan IgM/IgG-Rapid-Test, Wantai Rapid Test) and subsequently correlated to neutralization titers. The Wantai ELISA and the Elecsys provide the highest sensitivities in
this sample (98 and 95 percent respectively). Titers with the best overall quantitative correlation to the NT titer
were obtained with the Euroimmun IgG ELISA assay (Rho=0.759) and the Wantai ELISA assay (Rho=0.729).
An infection without fever and negative or weakly positive reactions in the Wantai Rapid test were negative
predictive factors for NT titers >1:200 (negative predictive value of 92 % and 92 % respectively, combination of
both 100 %)