Vero cell upstream bioprocess development for the production of viral vectors and vaccines
Autor
Kiesslich, Sascha
Kamen, Amine A.
Institución
Resumen
The Vero cell line is considered the most used continuous cell line for the production of viral vectors and
vaccines. Historically, it is the first cell line that was approved by the WHO for the production of human vaccines. Comprehensive experimental data on the production of many viruses using the Vero cell line can be found
in the literature. However, the vast majority of these processes is relying on the microcarrier technology. While
this system is established for the large-scale manufacturing of viral vaccine, it is still quite complex and labor
intensive. Moreover, scale-up remains difficult and is limited by the surface area given by the carriers. To
overcome these and other drawbacks and to establish more efficient manufacturing processes, it is a priority to
further develop the Vero cell platform by applying novel bioprocess technologies. Especially in times like the
current COVID-19 pandemic, advanced and scalable platform technologies could provide more efficient and
cost-effective solutions to meet the global vaccine demand.
Herein, we review the prevailing literature on Vero cell bioprocess development for the production of viral
vectors and vaccines with the aim to assess the recent advances in bioprocess development. We critically underline the need for further research activities and describe bottlenecks to imp