masterThesis
Determinación de los parámetros de trabajo de la lipasa de Pseudomonas aeruginosa aislada del fruto de la palma aceitera para su uso como biocatalizador en la producción de biodiésel
Fecha
2012Registro en:
Abbaszaadeh, A., Ghobadian, B., y Omidkhah, M. R. (2012). Current biodiesel production
technologies: A comparative review. Energy Conversion and Management.
Akoh, C. C., Sellappan, S., Fomuso, L. B., y Yankah, V. V. (2002). Enzymatic synthesis of
structured lipids. Lipid Biotechnology, , 433-478.
Akoh, C. C., Chang, S. W., Lee, G. C., y Shaw, J. F. (2007). Enzymatic approach to
biodiesel production. Journal of Agricultural and Food Chemistry, 22(55), 8995-9005.
Alcántara, A. R., y al, e. (2004). Resolution of racemic acids, esters and amines by candida
rugosa lipase in slightly hydrated organic media. Food Technology and Biotechnology,
(42), 343-354.
American Society for Testing Materials ASTM.ASTM D4175 - 09ae2 standard terminology
relating to petroleum, petroleum products, and lubricants. USA:
Ana, M. B. R., y Victor, P. H. (2007). Biodiesel synthesis by enzymatic transesterification
of palm oil with ethanol using lipases from several sources immobilized on Silica–
PVA composite. Energy Fuels, (27), 3689-3694.
Atadashi, I. M., Aroua, M. K., Abdul Azis, A. R., y Sulaiman, N. M. N. (2012). The effects
of water on biodiesel production and refining technologies: A review. Renewable and
Sustainable Energy Reviews, (16), 3456-3470.
Ateslier, Z. B. B., y Metin, K. (2006). Production and partial characterization of a novel
thermostable esterase from a thermophilic bacillus sp. Enzyme and Microbial
Technology, (38), 628-635.
Avellaneda, F. A. (2010). Producción y caracterización de biodiesel de palma y de aceite
reclicado mediante un proceso batch y un proceso continuo con un reactor helicoidal.
tesis doctoral. (). Tarragona: Universidad de Rovira.
Babu, V., y Goud, V. (2012). Biodiesel production from renewable feedstocks: Status and
opportunities. Renewable and Sustainable Energy Reviews, (16), 4763-4784.
Boocock, D. (1996). Fast one-phase oil-rich processes for the preparation of vegetable oil
methyl esters. Biomass and Bioenergy, , 43-50.
Borkar, P. S., Bodade, R. G., y Rao, S. R. (2009). Purification and characterization of
extracellular lipase from a new strain − pseudomonas aeruginosa SRT 9. Brazilian
Journal of Microbiology, (40), 358-366.
Cao, P. (2008). High-purity fatty acid methyl ester production from canola, soybean, palm,
and yellow grease lipids by means of a membrane reactor. Biomass and Bioenergy, ,
1028-1036.
Chartrain, M., Katz, L., y Marcin, C. (1993). Purification and characterization of a novel
bioconverting lipase from pseudomonas aeruginosa MB 5001. Enzyme Microbiology
Technology, (15), 575-580.
Chen, H. C. (2011). Continuous production of lipase-catalyzed biodiesel in a packed-bed
reactor: Optimization and enzyme reuse study. Journal of Biomedicine and
Biotechnology,
Consejo Nacional de Política Económica y Social. (2008). DOCUMENTO CONPES 3510
LINEAMIENTOS DE POLITICA PARA PROMOVER LA PRODUCCION
SOSTENIBLE DE BIOCOMBUSTIBLES EN COLOMBIA. (). Bogotá: Departamento
Nacional de Planeación.
Consorcio CUE. (2012). Evaluación del ciclo de vida de la cadena de producción de
biocombustibles en colombia. (). Medellin:
De, B. K. (1999). Enzymatic synthesis of fatty alcohol esters by alcoholysis. Journal of the
American Oil Chemists' Society, , 451-453.
Demirbas, A. (2005). Biodiesel production from vegetable oils via catalytic and non
catalytic supercritical methanol transesterification methods. Progress in Energy and
Combustion Science, , 466-487.
Demirbas, A. (2009). Progress and recent trends in biodiesel fuels. Energy Conversion and
Management, , 14-34.
Department of Bioinformatics & Biochemistry - Technische Universität Braunschweig.EC
3.1.1.3 - triacylglycerol lipase
Department of Bioinformatics & Biochemistry Technische Universität
Braunschweig.Sequence search
Devanesan, M. G. (2007). Transesterification of jatropha oil using immobilized
pseudomonas fluorescens. African Journal of Biotechnology, , 2497-2501.
Dharmsthiti, S. (1998). Lipase from pseudomonas aeruginosa LP602: Biochemical
properties and application for wastewater treatment. Journal of Industrial
Microbiology & Biotechnology, , 75-80.
Dominguez, P. (2002). Heptyl oleate synthesis as useful tool to discriminate between
lipases, proteases and other hydrolases in crude preparations. Enzyme and Microbial
Technology, , 283-288.
Federación Nacional de Biocombustibles de Colombia FEDEBIOCOMBUSTIBLES.
(2012). Cifras informativas del sector biocombustibles - biodiesel de palma de aceite
Federación Nacional de Cultivadores de Palma de Aceite FEDEPALMA.Federación
nacional de cultivadores de palma de aceite.
Federación Nacional de cultivadores de Palma FEDEPALMA. (2009). Programa de
biodiésel en colombia y su potenciial para la generación de energía eléctrica. ().
Bogotá.
Gao, Y. (2009). Optimization of transesterification conditions for the production of fatty
acid methyl ester (FAME) from chinese tallow kernel oil with surfactant-coated lipase.
Biomass and Bioenergy, , 277-282.
Gaur, R., Gupta, A., y Khare, S. K. (2008). Purification and characterization of lipase from
solvent tolerant pseudomonas aeruginosa PseA. Process Biochemistry, (43), 1040
1046.
Gilbert, E. J. (1991). Purification and properties of extracellular lipase from pseudomonas
aeruginosa EF2. Journal of General Microbiology, , 2223-2229.
Gupta, R., Gupta, N., y Rathi, P. (2004). Bacterial lipases: An overview of production,
purification and biochemical properties. Applied Microbiology Technology, (64), 763-
781.
Gutiérrez P, H., y De la Vara, R. (2008). Análisis y diseño de experimentos (Segunda ed.)
Mc Graw Hill.
Hama, S. (2011). Enzymatic packed-bed reactor integrated with glycerol-separating system
for solvent-free production of biodiesel fuel. Biochemical Engineering Journal, , 66-
71.
Harding, K. G., Dennis, J. S., von Blottnitz, H., y Harrison, S. T. L. (2008). A life-cycle
comparison between inorganic and biological catalysis for the production of biodiesel.
Journal of Cleaner Production, , 1368-1378.
Hasan, F., Shah, A. A., y Hameed, A. (2006). Industrial applications of microbial lipases.
Enzyme and Microbial Technology, , 235-251.
Instituto Colombiano de Normas Tecnicas y Certificación - ICONTEC. (2006). Norma
técnica colombiana NTC 5444 - BIODIESEL PARA USO EN MOTORES
DIESEL.ESPECIFICACIONES
Instituto Colombiano de Normas Tecnicas y Certificación - ICONTEC. (2011). Norma
técnica colombiana NTC 1438 - PETRÓLEO Y SUS DERIVADOS.COMBUSTIBLES
PARA MOTORES DIESEL
Itabaiana Jr., I. (2013). Towards a continuous flow environment for lipase-catalyzed
reactions. Journal of Molecular Catalysis B: Enzymatic, , 1-9.
Jegannathan, K. R., Abang, S., Poncelet, D., Chan, E. S., y Ravindra, P. (2008). Production
of biodiesel using immobilized lipase — a critical review. Critical Reviews
Biotechnology, (28), 253-264.
Kaieda, M., Samukawa, T., Kondo, A., y Fukuda, H. (2001). Effect of methanol and water
contents on production of biodiesel fuel from plant oil catalyzed by various lipases in a
solvent-free system. JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 91(1),
12-15.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of
bacteriophage T4. Nature, (227), 680-685.
Lee, D., Koh, Y., y Kim, K. (1999). Isolation and characterization of a thermophilic lipase
from bacillus thermoleovorans ID-1. FEMS Microbiology Letters, (179), 393-400.
Li, L. (2006). Lipase-catalyzed transesterification of rapeseed oils for biodiesel production
with a novel organic solvent as the reaction medium. Journal of Molecular Catalysis
B: Enzymatic, , 58-62
Linko, Y. Y. (1998). Biodegradable products bylipase biocatalysis. Journal of
Biotechnology, (66), 41-58
Marchetti, J. M. (2011). A summary of the available technologies for biodiesel production
based on a comparison of different feedstock’s properties. Process Safety and
Environmental Protection,
Mazo, P., Restrepo, G., y Rios, L. (2011). Alternative methods for fatty acid alkyl-esters
production: Microwaves, radio-frequency and ultrasound, biodiesel - feedstocks and processing technologies. In M. Stoytcheva, & G. Montero (Eds.), Biodiesel -
feedstocks and processing technologies (1st ed., pp. 269-288) InTech.
Mekhilef, S. (2011). A review on palm oil biodiesel as a source of renewable fuel.
Renewable and Sustainable Energy Reviews, , 1937-1949.
Ministerio de Minas y Energía. República de Colombia. (2012). Sector biocombustibles en
colombia. (). Bogotá:
Montenegro, C. (2005). Determinación del rendimiento de la reacción de
transesterificación para la obtención de biodiesel a partir de oleina de palma y
alcohol de caña, usando como catalizador la enzima novozym 435. trabajo de grado.
(Tesis, Universidad de la Sabana).
Murray, R. K., et al. (2010). HARPER. bioquímica ilustrada (28a ed.) McGraw Hill.
Nelson, D. L., y Cox, M. L. (2006). LEHNINGER. principios de bioquímica (Cuarta ed.)
Omega.
Oliveira, P. C. (2000). Immobilisation studies and catalytic properties of microbial lipase
onto styrene±divinylbenzene copolymer. Biochemical Engineering Journal, , 63-71.
Peña, F. (2006). Aprovechamiento de aceites residuales del proceso de fritura como
sustrato para el desarrollo de microorganismos productores de lipasas. (Trabajo de
grado para optar al titulo de Ingeniero de Producción Agroindustrial, Universidad de la
Sabana).
Peng, R. (2011). Co-expression of an organic solvent-tolerant lipase and its cognate foldase
of pseudomonas aeruginosa CS-2 and the application of the immobilized recombinant
lipase. Applied Biochemistry and Biotechnology, , 926-937.
Perancho, D. (2011). Tesis. estudio del funcionamiento de motores alternativos de
combustión interna mediante la utilización de combustibles alternativos. (). Cataluña:
Universitat Politècnica de Catalunya.
Pinto, A. C. (2005). Biodiesel: An overview. Journal of Brazilian Chemistry Society, ,
1313-1330.
Prescott, L. (2004). Microbiologia (Quinta ed.). Madrid: Mc. Graw Hill.
Prieto, R. E. (2006). Obtención de enzimas lipolíticas a partir de microrganismos aislados
del fruto de palma aceitera. informe proyecto COLCIENCIAS. (). Bogotá:
Prieto, R. E. (2012). Caracterización y mejoramiento de la acción de la lipasa de
pseudomonas aeruginosa para su utilización en la producción de biodiesel y
oleoquímicos. informe de proyecto COLCIENCIAS. (). Bogotá:
Rodrigues, R. (2010). Two step ethanolysis: A simple and efficient way to improve the
enzymatic biodiesel synthesis catalyzed by an immobilized–stabilized lipase from
thermomyces lanuginosus. Process Biochemistry, , 1268-1273.
Royon, D. (2007). Enzymatic production of biodiesel from cottonseed oil using t-butanol as
a solvent. Bioresources and Biotechnology, , 648-653.
Saeed, H. (2005). Purification and characterization of two extracellular lipases from
pseudomonas aeruginosa ps-x. Polish Journal of Microbiology, , 233-240.
Sha, S., y Gupta, M. N. (2007). Lipase catalyzed preparation of biodiesel from jatropha oil
in a solvent free system. Process Biochemistry, (42), 409-414.
Shah, S., y Gupta, N. (2008). The effect of ultrasonic pre-treatment on the catalytic activity
of lipases in aqueous and non-aqueous media. Chemistry Central Journal,
Sharma, R., Chisti, Y., y Banarjee, U. C. (2001). Production, purification, characterization,
and applications of lipases. Biotechnology Advances, (19), 627-662.
Sheng, F. L. (2011). Immobilization of pseudomonas cepacia lipase onto the electrospun
PAN nanofibrous membranes for transesterification reaction. Journal of Molecular
Catalysis B: Enzymatic, , 98-103.
Shie, C. J. (2003). Optimization of lipase-catalyzed biodiesel by response surface
methodology. Bioresource Technology, , 103-106
Shimada, Y. (2002). Enzymatic alcoholysis for biodiesel fuel production and enzymatic
alcoholysis for biodiesel fuel production and. Journal of Molecular Catalysis B:
Enzymatic, , 133-142.
Sim, J. H., Harun, A., y Bhatia, S. (2009). Effect of mass transfer and enzyme loading on
the biodiesel yield and reaction rate in the enzymatic transesterification of crude palm
oil. Energy & Fuels, (23), 4651-4658.
Sim, J. H., Kamruddin, A. H., y Bathia, S. (2010). The feasibility study of crude palm oil
transesterification at 30 °C operation. Bioresource Technology, (101), 8948-8954.
Stamatis, H., y Xenakis, A: Kolisis, F. N. (1999). Bioorganic reactions in microemulsions:
The case of lipases. Biotechnology Advances, , 293-318.
Stamenkovic, O. S., Velickovic, A. V., y Veljkovic. (2011). The production of biodiesel
from vegetable oils by ethanolysis: Current state and perspectives. Fuels, (90), 3141-
3145.
Swiss Institute of Bioinformatics.ENZYME entry: EC 3.1.1.3
Talukder, M. M. R., Wu, J. C., y Van Nguyen, T. B. (2009). Novozym 435 for production
of biodiesel from unrefined palm oil: Comparison of methanolysis methods. Journal of
Molecular Catalysis B: Enzymatic, (60), 106-112.
Talukder, M. R. (2010). Two-step lipase catalysis for production of biodiesel. Biochemical
Engineering Journal, , 207-212.
Tan, K. T., Lee, K. T., y Mohamed, A. R. (2009). Production of FAME by palm oil
transesterification via supercritical methanol technology. Biomass and Bioenergy, (33),
1096-1099.
Tan, T., Lu, J., y Nie, K. (2010). Biodiesel production with immobilized lipase: A review.
Biotechnology Advances, (28), 628-634.
Uscategui, Y. L. (2007). Evaluación de la cinética de crecimiento en función de la
concentración inicial de glucosa, y tiempo de inducción en la producción de enzimas
lipolíticas a partir de una bacteria aislada del fruto de palma africana (elaeis
guineensis). (Trabajo de grado para optar al titulo de Ingeniero de Producción
Agroindustrial, Universidad de la Sabana).
Van Gerpen, J. (2005). Biodiesel processing and production. Fuel Processing Technology, ,
1097-1107.
Watanabe, Y., Shimada, Y., Sugihara, A., y Tminaga, Y. (2002). Conversion of degummed
soybean oil to biodiesel fuel with immobilized candida antarctica lipase. Journal of
Molecular Catalysis B: Enzymatic, (17), 151-155.
Yapazan, E. (2008). Partial purification and characterization of lipase enzyme from a
pseudomonas strain. (Trabajo de grado - Maestría, İzmir Institute of Technology).
Yoshida, A. (2012). Improved performance of a packed-bed reactor for biodiesel
production through whole-cell biocatalysis employing a high-lipase-expression system.
Biochemical Engineering Journal, , 76-80
Zhao, X. (2007). An organic soluble lipase for water-free synthesis of biodiesel. Applied
Biochemistry and Biotechnology, , 236-243
Zor, T. a. S., Zvi. (1996). Linearization of the bradford protein assay increases its
sensitivity: Theoretical and experimental studies. Analytical Biochemistry, (236), 302–
308
256446
TE06199
Autor
Díaz Barrera, Luis Eduardo
Prieto Correa, Rosa Erlide
Institución
Resumen
Se utilizó lipasa de Pseudomona aeruginosa para transesterificación de aceite de palma Elaeis guineensis, utilizando diseños experimentales factoriales para las variables del proceso. El rendimiento molar de la reacción se calculó por cromatografía de gases, análisis de varianza y superficies de respuesta. Se utilizó extracto enzimático (EE) y lipasa parcialmente purificada (LPP), obteniendo el mejor resultado a pH 8, LPP, 5% v/v de agua y 10% v/v hexano aceite, relación estequiométrica 1:140, utilizando aceite de palma RBD a 54°C y agitación de 200 rpm durante 48 horas. Se encontró que la lipasa presenta resistencia a altas concentraciones de metanol y bajas concentraciones de hexano. El rendimiento molar promedio a partir de la optimización del proceso incrementó 16 veces.