dc.date.accessioned | 2020-10-23T14:11:33Z | |
dc.date.accessioned | 2022-09-23T13:58:02Z | |
dc.date.available | 2020-10-23T14:11:33Z | |
dc.date.available | 2022-09-23T13:58:02Z | |
dc.date.created | 2020-10-23T14:11:33Z | |
dc.date.issued | 2019-03-16 | |
dc.identifier | 1596-5996 | |
dc.identifier | https://www.ajol.info/index.php/tjpr/article/view/184711 | |
dc.identifier | http://hdl.handle.net/10818/43796 | |
dc.identifier | 10.4314/tjpr.v17i11.13 | |
dc.identifier | 1596-9827 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3473208 | |
dc.description.abstract | Purpose: To optimize the L-asparaginase activity of Actinobacteria isolated from Guaviare river sediments in Colombia.
Methods: Actinobacterial strains were evaluated for their L-asparaginase activity using phenol red plates and Nessler’s assays. Strains with L-asparaginase activity were identified based on 16S ribosomal rRNA sequencing, and a central composite design was used to study nutritional and growth factors that could improve L-asparaginase activity. L-asparaginase protein was detected using western blotting and the cytotoxicity of L-asparaginase preparations was evaluated against MDA-MB231 and L929 cell lines.
Results: Kitasatospora atroaurantiaca, Streptomyces griseoluteus, and Streptomyces panaciradicis were cultured in medium with lactose as a carbon source and a combination of asparagine and malt extract as nitrogen sources. These strains showed L-asparaginase activities of 29.4, 114.06, and 34.08 U/mg, respectively, and half-maximal inhibitory concentration (IC50) values of 25.61 ± 2.15, 8.18 ± 1.61, and 165.29 ± 1.06 ppm, respectively, against MDA-MB 231 cells. Western blotting analysis revealed the presence of an L-asparaginase monomer with a molecular weight of 37 kDa.
Conclusion: Kitasatospora atroaurantiaca, Streptomyces griseoluteus, and Streptomyces Panaciradicis produce L-asparaginases with low L-glutaminase activity and promising cytotoxic activity and thus may be useful for the management of acute lymphoblastic leukemia. | |
dc.language | eng | |
dc.publisher | Tropical Journal of Pharmaceutical Research | |
dc.relation | Trop J Pharm Res, November 2018; 17(11): 2199 | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.rights | openAccess | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | |
dc.source | Universidad de La Sabana | |
dc.source | Intellectum Repositorio Universidad de La Sabana | |
dc.subject | L-asparaginase | |
dc.subject | Streptomyces | |
dc.subject | Kitasatospora | |
dc.subject | Optimization | |
dc.subject | Cytotoxicity | |
dc.title | Optimization of L-asparaginase activity of Actinobacteria isolated from Guaviare river sediments in Colombia | |
dc.type | journal article | |