Significant enzymatic activities in the residues hydrolysis of the sugar cane harvest

dc.contributorUniversidad Nacional de Colombia, Sede Medellín
dc.creatorSalcedo Mendoza, Jairo Guadalupe
dc.creatorLuz Marina, Flórez Pardo
dc.creatorLópez Galán, Jorge Enrique
dc.date.accessioned2021-11-04T17:04:34Z
dc.date.accessioned2022-09-22T18:37:45Z
dc.date.available2021-11-04T17:04:34Z
dc.date.available2022-09-22T18:37:45Z
dc.date.created2021-11-04T17:04:34Z
dc.date.issued2019-07
dc.identifier127353
dc.identifierhttps://hdl.handle.net/10614/13402
dc.identifier10.15446/dyna.v86n210.75286
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3455496
dc.description.abstractIn the production of ethanol from agroindustrial crop residues, one of the critical stages in the process is the conversion of lignocellulosic material to simple sugars, which can be done chemically or enzymatically. In this research, the enzymatic activities of commercial enzymes were evaluated for their influence on the degradation of lignocellulosic materials from sugar cane harvest residues (leaves and top cane). Eight substrates were pretreated with different delignification methods. Likewise, five enzymatic preparations were configured. An analysis of the enzyme-substrate interactions was conducted through fuzzy system analysis. The results showed regions of maximum enzymatic activity for residues of the sugarcane harvest, between 20-30 Filter Paper Units (FPU) /mL values lower than 500 pNPG (p-Nitrofenol-α-D-Glucopyranoside) U / mL of activity beta-glucosidase and hemicellulase activity between 50 and 70 IU / mL, confirming that the use of large amounts of cellulolytic enzymes is not necessary
dc.description.abstractEn la producción de etanol a partir de residuos agroindustriales, una de las etapas críticas en el proceso es la conversión del material lignocelulósico a azúcares simples, que puede realizarse química o enzimáticamente. En esta investigación, se evaluó la influencia de las actividades enzimáticas de las enzimas comerciales para degradar materiales de residuos de cosecha de la caña de azúcar (hojas y cogollos). Ocho sustratos fueron pretratados con diferentes métodos de deslignificación, con cinco preparaciones enzimáticas. Se utilizó un análisis de las interacciones enzima-sustrato, a través del análisis del sistema difuso. Los resultados mostraron regiones de actividad enzimática entre 20-30 FPU / mL y valores inferiores a 500 pNPG U / mL de actividad beta-glucosidasa y para actividad hemicelulasa entre 50 y 70 IU / mL, confirmando que el uso de grandes cantidades de enzimas celulolíticas no es necesario
dc.languageeng
dc.publisherUniversidad Nacional de Colombia, Sede Medellín
dc.publisherMedellìn
dc.relationVolumen 86, número 210 (2019)
dc.relation41
dc.relation210
dc.relation35
dc.relation86
dc.relationSalcedo Mendoza, J.G., Florez Pardo, L.M., López Galán, J. E. (2019). Significant enzymatic activities in the residues hydrolysis of the sugar cane harvest. Revista DYNA, (Vol. 86 (210), pp. 35-41. https://doi.org/10.15446/dyna.v86n210.75286
dc.relationDYNA
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dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
dc.rightsDerechos reservados - Universidad Nacional de Colombia, Sede Medellín, 2019
dc.titleSignificant enzymatic activities in the residues hydrolysis of the sugar cane harvest
dc.typeArtículo de revista


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