Efecto antibacteriano de una nanoemulsión de ftalocianina de aluminio clorada sobre periodontopatógenos relevantes en el paciente diabético tipo 2: Estudio in vitro

dc.contributorHerrera Sandoval, Laura-Viviana
dc.contributorMéndez Díaz, Luz-Mery
dc.contributorLeal Pinto, Sandra-Milena
dc.creatorLloreda Rey, Laura-Patricia
dc.date.accessioned2020-02-06T23:06:41Z
dc.date.available2020-02-06T23:06:41Z
dc.date.created2020-02-06T23:06:41Z
dc.date.issued2019-09-16
dc.identifierT 86.19 L567e
dc.identifierhttps://repositorio.udes.edu.co/handle/001/4426
dc.description.abstractLa enfermedad periodontal (EP) y la diabetes mellitus (DM) son enfermedades crónicas con una relación bidireccional. El tratamiento convencional para la EP es altamente invasivo, consiste en la remoción mecánica de agentes microbianos de las superficies dentales. Investigaciones han propuesto el uso de la terapia fotodinámica (TFD) como modalidad terapéutica prometedora para el manejo de infecciones de la cavidad bucal. En este sentido, el objeto general fue determinar el efecto antimicrobiano in vitro de una nanoemulsión de Ftalocianina de Aluminio Clorada (NE-PcAlCl ) en combinación con la terapia fotodinámica sobre el crecimiento de periodontopatógenos prevalentes en la enfermedad periodontal del paciente diabético tipo 2. Metodológicamente, se evaluó el efecto antibacteriano de NE-PcAlCl y PcAlCl-libre contra Prevotella intermedia, Porphyromonas gingivalis y Aggregatibacter actinomycetemcomitans en condiciones de anaerobiosis mediante el método de microdilución. La actividad citotóxica fue evaluada en células Vero por el ensayo colorimétrico usando la sal de tetrazolio MTT. Para la TFD se utilizó una fuente de luz LED a 4.83 julios/cm2 por 2 minutos. Controles sin irradiación fueron probados bajo las mismas condiciones. Los resultados demostraron efecto antibacteriano de la NE-PcAlCl contra P. intermedia con CMI de 0,63 μM luego de la TFD, para el caso de P. gingivalis y A. actinomycetemcomitans no se encontró efecto de la NE-PcAlCl en las concentraciones evaluadas (> 20 μM) antes y después de la TFD. Por otro lado, la PcAlCl-libre y en nanoemulsión mostraron ser fototóxicas sobre células Vero (CC50 de 0,155 y 0,09 μM, respectivamente) sin registrar diferencias significativas entre ellas (p<0.05). En este sentido, los resultados obtenidos sugieren el potencial uso de la TFD en combinación con la NE-PcAlCl para la inhibición de P. intermedia, periodontopatógeno altamente prevalente en el paciente diabético tipo 2.
dc.description.abstractPeriodontal disease (PD) and diabetes mellitus (DM) are chronic diseases with a bidirectional relationship. Conventional treatment for PE is highly invasive and involves the mechanical removal of bacterial agents of the dental surface. Investigations have proposed the use of photodynamic therapy (PDT) as a promising therapeutic modality for the management of infections of the oral cavity. In this sense, the general purpose was to determine the in vitro antimicrobial effect of a Chlorinated Aluminum Phthalocyanine (NE-PcAlCl) nanoemulsion in combination with photodynamic therapy on the growth of periodontal disease in a type 2 diabetic patient. Methodologically, the antibacterial effect of NE-PcAlCl and free-PcAlCl were evaluated against Prevotella intermedia, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in anaerobic conditions by the microdilution method. Cytotoxic activity was evaluated in Vero cells by colorimetric assay using the MTT tetrazolium salt. For the TFD an LED light source at 4.83 joules /cm2 was used for 2 minutes. Controls without irradiation were tested in the same conditions. The results demonstrated the antibacterial effect of NE-PcAlCl against P. intermedia with MIC of 0.63 μM after PDT. In the case of P. gingivalis and A. actinomycetemcomitans, no effect of NE-PcAlCl was found at the concentrations evaluated (> 20 μM) before and after PDT. On the other hand, PcAlCl-free and nanoemulsion showed to be phototoxic on Vero cells (CC50 of 0.155 and 0.09 μM, respectively) without registering significant differences between them (p <0.05). In this sense, the results obtained suggest the potential use of PDT in combination with NE-PcAlCl for the inhibition of P. intermedia, periodontopathogen highly prevalent in the type 2 diabetic patient
dc.languagespa
dc.publisherBucaramanga : Universidad de Santander, 2019
dc.publisherFacultad Ciencias de la Salud
dc.publisherMaestría en Investigación en Enfermedades Infecciosas
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dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)
dc.rightshttps://creativecommons.org/licenses/by-nc/4.0/
dc.rightsDerechos Reservados - Universidad de Santander, 2019
dc.titleEfecto antibacteriano de una nanoemulsión de ftalocianina de aluminio clorada sobre periodontopatógenos relevantes en el paciente diabético tipo 2: Estudio in vitro
dc.typeTrabajo de grado - Maestría


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