The Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells

dc.creatorTaylor, J.
dc.creatorPereyra, Andrea Soledad
dc.creatorZhang, T.
dc.creatorMessi, M. L.
dc.creatorWang, Z. M.
dc.creatorHereñú, Claudia Beatriz
dc.creatorKuan, P. F.
dc.creatorDelbono, O.
dc.date2014-06-16
dc.date2019-11-12T15:19:08Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/85405
dc.identifierissn:0021-9525
dc.descriptionVoltage-gated calcium channel (Cav) β subunits are auxiliary subunits to Cavs. Recent reports show Cavβ subunits may enter the nucleus and suggest a role in transcriptional regulation, but the physiological relevance of this localization remains unclear. We sought to define the nuclear function of Cavβ in muscle progenitor cells (MPCs). We found that Cavβ1a is expressed in proliferating MPCs, before expression of the calcium conducting subunit Cav1.1, and enters the nucleus. Loss of Cavβ1a expression impaired MPC expansion in vitro and in vivo and caused widespread changes in global gene expression, including up-regulation of myogenin. Additionally, we found that Cavβ1a localizes to the promoter region of a number of genes, preferentially at noncanonical (NC) E-box sites. Cavβ1a binds to a region of the Myog promoter containing an NC E-box, suggesting a mechanism for inhibition of myogenin gene expression. This work indicates that Cavβ1a acts as a Cav-independent regulator of gene expression in MPCs, and is required for their normal expansion during myogenic development.
dc.descriptionInstituto de Investigaciones Bioquímicas de La Plata
dc.formatapplication/pdf
dc.format829-846
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/3.0/
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)
dc.subjectCiencias Médicas
dc.subjectQuímica
dc.subjectCav, voltage-gated calcium channel
dc.subjectChIP, chromatin immunoprecipitation
dc.subjectDM, differentiation medium
dc.subjectEC, excitation- contraction
dc.subjectEMSA, electrophoretic mobility shift assay
dc.subjectGK, guanylate kinase
dc.subjectGM, growth medium
dc.subjectH&E, hematoxylin & eosin
dc.subjectLMB, leptomycin-B
dc.subjectMPC, muscle progenitor cell
dc.subjectNC, noncanonical
dc.subjectNLS, nuclear localization sequence
dc.subjectRAd, recombinant adenoviral
dc.subjectSH3, Src homology 3
dc.subjectTSS, transcription start site
dc.titleThe Ca<sub>v</sub>β<sub>1a</sub> subunit regulates gene expression and suppresses myogenin in muscle progenitor cells
dc.typeArticulo
dc.typeArticulo


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