| dc.creator | Riccillo, Pablo Miguel | |
| dc.creator | Muglia, Cecilia Isabel | |
| dc.creator | Bruijn, Frans J. de | |
| dc.creator | Roe, Andrew J. | |
| dc.creator | Booth, Ian R. | |
| dc.creator | Aguilar, Orlando Mario | |
| dc.date | 2000 | |
| dc.date | 2019-10-30T14:57:45Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/84378 | |
| dc.identifier | issn:0021-9193 | |
| dc.description | The isolation of rhizobial strains which exhibit an intrinsic tolerance to acidic conditions has been reported and has facilitated studies on the basic mechanisms underlying acid tolerance. <i>Rhizobium tropici</i> strain CIAT899 displays a high intrinsic tolerance to acidity and therefore was used in this work to study the molecular basis of bacterial responses to acid conditions and other environmental stresses. We generated a collection of <i>R. tropici</i> CIAT899 mutants affected in acid tolerance using Tn<i>5-luxAB</i> mutagenesis, and one mutant strain (CIAT899-13T2), which fails to grow under acid conditions, was characterized in detail. Strain CIAT899-13T2 was found to contain a single Tn<i>5-luxAB</i> insertion in a gene showing a high degree of similarity with the <i>Escherichia coli gshB</i> gene, encoding the enzyme glutathione synthetase. Intracellular potassium pools and intracellular pH levels were found to be lower in the mutant than in the parent. The glutathione-deficient mutant was shown to be sensitive to weak organic acids, osmotic and oxidative stresses, and the presence of methylglyoxal. Glutathione restores responses to these stresses almost to wild-type levels. Our data show that in <i>R. tropici</i> the production of glutathione is essential for growth in extreme environmental conditions. The mutant strain CIAT899-13T2 induced effective nodules; however, it was found to be outcompeted by the wild-type strain in coinoculation experiments. | |
| dc.description | Facultad de Ciencias Exactas | |
| dc.description | Instituto de Biotecnologia y Biologia Molecular | |
| dc.format | application/pdf | |
| dc.format | 1748-1753 | |
| dc.language | en | |
| dc.rights | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
| dc.rights | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
| dc.subject | Ciencias Exactas | |
| dc.subject | Rhizobium tropici | |
| dc.subject | acidity | |
| dc.subject | glutathione | |
| dc.title | Glutathione is involved in environmental stress responses in Rhizobium tropici, including acid tolerance | |
| dc.type | Articulo | |
| dc.type | Articulo | |
