Articulo
Disruption of dTDP-rhamnose biosynthesis modifies lipopolysaccharide core, exopolysaccharide production, and root colonization in Azospirillum brasilense
Autor
Jofré, Edgardo
Lagares, Antonio
Mori, Gladys
Institución
Resumen
The interaction between <i>Azospirillum brasilense</i> and plants is not fully understood, although several bacterial surface components like exopolysaccharides (EPS), flagella, and capsular polysaccharides are required for attachment and colonization. While in other plant-bacteria associations (<i>Rhizobium</i>-legume, <i>Pseudomonas</i>-potato), lipopolysaccharides (LPS) play a key role in the establishment of an effective association, their role in the root colonization by Azospirillum had not been determined. In this study, we isolated a Tn5 mutant of <i>A. brasilense</i> Cd (EJ1) with an apparently modified LPS core structure, non-mucoid colony morphology, increased EPS production, and affected in maize root colonization. A 3790-bp region revealed the presence of three complete open reading frames designated <i>rmlC</i>, <i>rmlB</i> and <i>rmlD</i>. The beginning of a fourth open reading frame was found and designated rmlA. These genes are organized in a cluster which shows homology to the cluster involved in the synthesis of dTDP-rhamnose in other bacteria. Additionally, the analysis of the monosaccharide composition of LPSs showed a diminution of rhamnose compared to the wild-type strain. Facultad de Ciencias Exactas Instituto de Biotecnologia y Biologia Molecular