| dc.creator | Serra, Diego Omar | |
| dc.creator | Lücking, Genia | |
| dc.creator | Weiland, Florian | |
| dc.creator | Schulz, Stefan | |
| dc.creator | Görg, Angelika | |
| dc.creator | Yantorno, Osvaldo Miguel | |
| dc.creator | Ehling Schulz, Monika | |
| dc.date | 2008 | |
| dc.date | 2019-10-28T15:13:52Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/84205 | |
| dc.identifier | issn:1615-9853 | |
| dc.description | Proteome analysis was combined with whole-cell metabolic fingerprinting to gain insight into the physiology of mature biofilm in <i>Bordetella pertussis</i>, the agent responsible for whooping cough. Recent reports indicate that <i>B. pertussis</i> adopts a sessile biofilm as a strategy to persistently colonize the human host. However, since research in the past mainly focused on the planktonic lifestyle of <i>B. pertussis</i>, knowledge on biofilm formation of this important human pathogen is still limited. Comparative studies were carried out by combining 2-DE and Fourier transform infrared (FT-IR) spectroscopy with multivariate statistical methods. These complementary approaches demonstrated that biofilm development has a distinctive impact on <i>B. pertussis</i> physiology. Results from MALDI-TOF/MS identification of proteins together with results from FT-IR spectroscopy revealed the biosynthesis of a putative acidic-type polysaccharide polymer as the most distinctive trait of <i>B. pertussis</i> life in a biofilm. Additionally, expression of proteins known to be involved in cellular regulatory circuits, cell attachment and virulence was altered in sessile cells, which strongly suggests a significant impact of biofilm development on <i>B. pertussis</i> pathogenesis. In summary, our work showed that the combination of proteomics and FT-IR spectroscopy with multivariate statistical analysis provides a powerful tool to gain further insight into bacterial lifestyles. | |
| dc.description | Facultad de Ciencias Exactas | |
| dc.description | Centro de Investigación y Desarrollo en Fermentaciones Industriales | |
| dc.format | application/pdf | |
| dc.format | 4995-5010 | |
| dc.language | en | |
| dc.rights | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
| dc.rights | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
| dc.subject | Ciencias Exactas | |
| dc.subject | Biofilm | |
| dc.subject | Bordetella pertussis | |
| dc.subject | FT-IR spectroscopy | |
| dc.title | Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis | |
| dc.type | Articulo | |
| dc.type | Articulo | |
