Human embryonic stem cells and derived contractile embryoid bodies are susceptible to Coxsakievirus B infection and respond to interferon Iβ treatment

dc.creatorScassa, María E.
dc.creatorJaquenod De Giusti, Carolina
dc.creatorQuesta, María
dc.creatorPrêtre, Gabriela
dc.creatorVidela Richardson, Guillermo A.
dc.creatorBluguermann, Carolina
dc.creatorRomorini, Leonardo
dc.creatorFerrer, María Florencia
dc.creatorSevlever, Gustavo E.
dc.creatorMiriuka, Santiago Gabriel
dc.creatorGómez, Ricardo Martín
dc.date2011
dc.date2019-10-28T12:33:39Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/84131
dc.identifierissn:1873-5061
dc.descriptionWe studied the susceptibility of human embryonic stem cells and derived contractile embryoid bodies from WAO9, HUES-5 and HUES-16 cell lines to Coxsackievirus B infection. After validating stem cell-like properties and cardiac phenotype, Coxsackievirus B receptors CAR and DAF, as well as type I interferon receptors were detected in all cell lines and differentiation stages studied. Real-time PCR analysis showed that CAR mRNA levels were 3.4-fold higher in undifferentiated cells, while DAF transcript levels were 2.78-fold more abundant in differentiated cultures (P<0.05). All cell lines were susceptible to Coxsackievirus serotypes B1-5 infection as shown by RT-PCR detection of viral RNA, immunofluorescence detection of viral protein and infectivity titration of cell culture supernatants resulting in cell death. Supernatants infectivity titers 24-48h post-infection ranged from 10<SUP>5</SUP>-10<SUP>6</SUP> plaque forming units (PFU)/ml, the highest titers were detected in undifferentiated cells. Cell viability detected by a colorimetric assay, showed inverse correlation with infectivity titers of cell culture supernatants. Treatment with 100 U of interferon Iβ significantly reduced viral replication and associated cell death during a 24-48 h observation period, as detected by reduced infectivity titers in the supernatants and increased cell viability by a colorimetric assay, respectively. We propose human embryonic stem cell and derived contractile embryoid bodies as a valid model to study cardiac Coxsackievirus B infection.
dc.descriptionInstituto de Biotecnologia y Biologia Molecular
dc.descriptionFacultad de Ciencias Exactas
dc.formatapplication/pdf
dc.format13-22
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.subjectCiencias Exactas
dc.subjectCiencias Médicas
dc.subjectCoxsackievirus B infection
dc.subjecthuman embryonic stem cell
dc.subjectderived contractile embryoid bodies
dc.titleHuman embryonic stem cells and derived contractile embryoid bodies are susceptible to Coxsakievirus B infection and respond to interferon Iβ treatment
dc.typeArticulo
dc.typeArticulo


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