| dc.creator | Villa Abrille, María Celeste | |
| dc.creator | Vila Petroff, Martín Gerardo | |
| dc.creator | Aiello, Ernesto Alejandro | |
| dc.date | 2006-12-01 | |
| dc.date | 2019-10-10T13:35:34Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/83019 | |
| dc.identifier | issn:0022-3751 | |
| dc.description | Perforated whole-cell configuration of patch clamp was used to determine the contribution of the electrogenic Na+/ HCO3- cotransport (NBC) on the shape of the action potential in cat ventricular myocytes. Switching from Hepes to HCO3 - buffer at constant extracellular pH (pHo) hyperpolarized resting membrane potential (RMP) by 2.67 ± 0.42 mV (n = 9, P < 0.05). The duration of action potential measured at 50% of repolarization time (APD50) was 35.8 ± 6.8% shorter in the presence of HCO3- than in its absence (n =9, P < 0.05). The anion blocker SITS prevented and reversed the HCO3--induced hyperpolarization and shortening of APD. In addition, no HCO3-induced hyperpolarization and APD shortening was observed in the absence of extracellular Na+. Quasi-steady-state currents were evoked by 8 s duration voltage-clamped ramps ranging from -130 to +30 mV. A novel component of SITS-sensitive current was observed in the presence of HCO3-. The HCO3--sensitive current reversed at -87 ± 5 mV (n =7), a value close to the expected reversal potential of an electrogenic Na+/HCO3- cotransport with a HCO3-: Na+ stoichiometry ratio of 2: 1. The above results allow us to conclude that the cardiac electrogenic Na+/HCO3- cotransport has a relevant influence on RMP and APD of cat ventricular cells. | |
| dc.description | Facultad de Ciencias Médicas | |
| dc.format | application/pdf | |
| dc.format | 819-829 | |
| dc.language | en | |
| dc.rights | http://creativecommons.org/licenses/by/4.0/ | |
| dc.rights | Creative Commons Attribution 4.0 International (CC BY 4.0) | |
| dc.subject | Ciencias Médicas | |
| dc.subject | cat ventricular myocytes | |
| dc.subject | electrogenic Na+/HCO3− cotransport | |
| dc.title | The electrogenic Na+/HCO3- cotransport modulates resting membrane potential and action potential duration in cat ventricular myocytes | |
| dc.type | Articulo | |
| dc.type | Articulo | |
