| dc.creator | Gazzaniga, Silvina | |
| dc.creator | Bravo, Alicia I. | |
| dc.creator | Guglielmotti, Angelo | |
| dc.creator | Van Rooijen, Nico | |
| dc.creator | Maschi, Fabricio Alejandro | |
| dc.creator | Vecchi, Annunciata | |
| dc.creator | Mantovani, Alberto | |
| dc.creator | Mordoh, José | |
| dc.creator | Wainstok, Rosa | |
| dc.date | 2007 | |
| dc.date | 2019-10-10T15:26:31Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/83061 | |
| dc.identifier | issn:0022-202X | |
| dc.description | Chemokines such as monocyte chemoattractant protein (MCP)-1 are key agonists that attract macrophages to tumors. In melanoma, it has been previously shown that variable levels of MCP-1/CCL2 appear to correlate with infiltrating macrophages and tumor fate, with low to intermediate levels of the chemokine contributing to melanoma development. To work under such conditions, a poorly tumorigenic human melanoma cell line was transfected with an expression vector encoding MCP-1. We found that M2 macrophages are associated to MCP-1<SUP>+</SUP> tumors, triggering a profuse vascular network. To target the protumoral macrophages recruitment and reverting tumor growth promotion, clodronate-laden liposomes (Clod-Lip) or bindarit were administered to melanoma-bearing mice. Macrophage depletion after Clod-Lip treatment induced development of smaller tumors than in untreated mice. Immunohistochemical analysis with an anti-CD31 antibody revealed scarce vascular structures mainly characterized by narrow vascular lights. Pharmacological inhibition of MCP-1 with bindarit also reduced tumor growth and macrophage recruitment, rendering necrotic tumor masses. We suggest that bindarit or Clod-Lip abrogates protumoral-associated macrophages in human melanoma xenografts and could be considered as complementary approaches to antiangiogenic therapy. | |
| dc.description | Facultad de Ciencias Veterinarias | |
| dc.format | application/pdf | |
| dc.format | 2031-2041 | |
| dc.language | en | |
| dc.rights | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
| dc.rights | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
| dc.subject | Ciencias Veterinarias | |
| dc.subject | Ciencias Médicas | |
| dc.subject | melanoma | |
| dc.subject | tumor | |
| dc.title | Targeting tumor-associated macrophages and inhibition of MCP-1 reduce angiogenesis and tumor growth in a human melanoma xenograft | |
| dc.type | Articulo | |
| dc.type | Articulo | |
