Calbindin D28k Expression and the Absence of Apoptosis in the Cerebellum of Solatium bonariense L-lntoxicated Bovines

dc.creatorVerdes, J. M.
dc.creatorMoraña, J. A.
dc.creatorBattes, D.
dc.creatorGutiérrez, F.
dc.creatorGuerrero, F.
dc.creatorGoicoa, A.
dc.creatorFidalgo, L. E.
dc.creatorBarbeito, Claudio Gustavo
dc.creatorZanuzzi, Carolina Natalia
dc.creatorPortiansky, Enrique Leo
dc.creatorGimeno, Eduardo Juan
dc.date2010
dc.date2019-10-02T17:55:50Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/82543
dc.identifierissn:03009858
dc.description<i>Solanum bonariense</i> intoxication is characterized by cerebellar neuronal vacuolation, degeneration, and necrosis. Cerebellar Purkinje cells seem especially susceptible, but more research is needed to determine the pathogenesis of neuronal necrosis and the mechanism of Purkinje cell susceptibility. Calbindin D28k (CbD28k) is highly expressed in Purkinje cells and has been used as a marker for normal and degenerative Purkinje cells. The goal of this study was to describe <i>S bonariense</i>-induced disease by ascertaining Purkinje cell-specific degenerative changes using CbD28k expression and to correlate this with apoptosis in Purkinje cells, as determined using TUNEL (transferase-mediated dUTP-biotin nick end-labeling) and ultrastructural changes. In all cases, an increase in both dose and duration of <i>S bonariense</i> intoxication resulted in a decrease in the number of Purkinje cells. CbD28k immunohistochemistry was an excellent marker for Purkinje cells because immunoreactivity did not change in normal or degenerative tissues. This finding suggests that excessive calcium excitatory stimulation does not induce rapid neuronal degeneration and death. As found in previous studies, TUNEL tests and electron microscopy suggest that Purkinje cell degeneration and death are not occurring via an apoptotic process. These findings suggest that <i>S bonariense</i> poisoning induces progressive Purkinje cell death that is not mediated by excitotoxicity or apoptotic activation.
dc.descriptionFacultad de Ciencias Veterinarias
dc.formatapplication/pdf
dc.format569-572
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.subjectCiencias Veterinarias
dc.subjectApoptosis
dc.subjectCalbindin D28k
dc.subjectCattle
dc.subjectCerebellar degeneration
dc.subjectExcitotoxicity
dc.subjectPlant poisoning
dc.subjectPurkinje cells
dc.titleCalbindin D28k Expression and the Absence of Apoptosis in the Cerebellum of Solatium bonariense L-lntoxicated Bovines
dc.typeArticulo
dc.typeArticulo


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